Document Detail


Salmonella testing of pooled pre-enrichment broth cultures for screening multiple food samples.
MedLine Citation:
PMID:  4553135     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A method has been described for testing multiple food samples for Salmonella without loss in sensitivity. The method pools multiple pre-enrichment broth cultures into single enrichment broths. The subsequent stages of the Salmonella analysis are not altered. The method was found applicable to several dry food materials including nonfat dry milk, dried egg albumin, cocoa, cottonseed flour, wheat flour, and shredded coconut. As many as 25 pre-enrichment broth cultures were pooled without apparent loss in the sensitivity of Salmonella detection as compared to individual sample analysis. The procedure offers a simple, yet effective, way to increase sample capacity in the Salmonella testing of foods, particularly where a large proportion of samples ordinarily is negative. It also permits small portions of pre-enrichment broth cultures to be retained for subsequent individual analysis if positive tests are found. Salmonella testing of pooled pre-enrichment broths provides increased consumer protection for a given amount of analytical effort as compared to individual sample analysis.
Authors:
W R Price; R A Olsen; J E Hunter
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Applied microbiology     Volume:  23     ISSN:  0003-6919     ISO Abbreviation:  Appl Microbiol     Publication Date:  1972 Apr 
Date Detail:
Created Date:  1972-06-22     Completed Date:  1972-06-22     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  7605802     Medline TA:  Appl Microbiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  679-82     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Bacteriological Techniques*
Cacao
Coloring Agents
Culture Media*
Egg White
Food Microbiology*
Lactose
Milk
Salmonella / growth & development,  isolation & purification*
Chemical
Reg. No./Substance:
0/Coloring Agents; 0/Culture Media; 63-42-3/Lactose
Comments/Corrections

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