Document Detail


Salmonella pathogenicity island 1 differentially modulates bacterial entry to dendritic and non-phagocytic cells.
MedLine Citation:
PMID:  20201987     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Salmonella enterica serovar Typhimurium can enter non-phagocytic cells, such as intestinal epithelial cells, by virtue of a Type Three Secretion System (TTSS) encoded in the Salmonella Pathogenicity Island 1 (SPI-1), which translocates bacterial effector molecules into the host cell. Salmonella can also be taken up by dendritic cells (DCs). Although the role of SPI-1 in non-phagocytic cell invasion is well established, its contribution to invasion of phagocytic cells has not been evaluated. Here, we have tested the invasive capacity of a S. Typhimurium strain lacking a key component of its TTSS-1 (DeltaInvC) leading to defective translocation of SPI-1-encoded effectors. Whereas this mutant Salmonella strain was impaired for invasion of non-phagocytic cells, it was taken up by DCs at a significantly higher rate than wild-type Salmonella. Similar to wild-type Salmonella, the DeltaInvC mutant strain retained the capacity to avoid antigen presentation to T cells. However, mice infected with the DeltaInvC mutant strain showed higher survival rate and reduced organ colonization. Our data suggest that, besides promoting phagocytosis by non-phagocytic cells, SPI-1 modulates the number of bacteria that enters DCs. The SPI-1 could be considered not only as an inducer of epithelial cell invasion but as a controller of DC entry.
Authors:
Susan M Bueno; Aniela Wozniak; Eduardo D Leiva; Sebastián A Riquelme; Leandro J Carreño; Wolf-Dietrich Hardt; Claudia A Riedel; Alexis M Kalergis
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-02-26
Journal Detail:
Title:  Immunology     Volume:  130     ISSN:  1365-2567     ISO Abbreviation:  Immunology     Publication Date:  2010 Jun 
Date Detail:
Created Date:  2010-06-18     Completed Date:  2010-07-09     Revised Date:  2013-05-30    
Medline Journal Info:
Nlm Unique ID:  0374672     Medline TA:  Immunology     Country:  England    
Other Details:
Languages:  eng     Pagination:  273-87     Citation Subset:  IM    
Affiliation:
Millennium Nucleus on Immunology and Immunotherapy, Departamento de Genética Molecular y Microbiología, Facultad de Ciencias Biológicas, Pontificia Universidad Católica de Chile, Santiago, Chile. sbueno@bio.puc.cl
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MeSH Terms
Descriptor/Qualifier:
Animals
Antigen Presentation / genetics,  immunology
Bacterial Proteins / genetics,  immunology*,  metabolism
Bacterial Translocation / genetics,  immunology*
Dendritic Cells / immunology*,  metabolism,  microbiology
Epithelial Cells / immunology*,  metabolism,  microbiology
Gene Deletion
Mice
Mice, Transgenic
Phagocytosis / genetics,  immunology
Salmonella typhimurium / genetics,  immunology*,  metabolism,  pathogenicity*
T-Lymphocytes / immunology,  metabolism,  microbiology
Chemical
Reg. No./Substance:
0/Bacterial Proteins; 0/Spi1 protein, Salmonella
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