Document Detail


Salmonella must be viable in order to attach to the surface of prepared vegetable tissues.
MedLine Citation:
PMID:  18399976     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
AIMS: The aims of the current study were to explore the site of bacterial attachment to vegetable tissues and to investigate the hypothesis that Salmonella must be living in order to attach to this site(s). METHODS AND RESULTS: Scanning electron micrographs of intact potato cells showed that Salm. serotype Typhimurium attached to cell-wall junctions; suggesting a high-level of site selectivity. Inactivation of Salm. Typhimurium using heat, ethanol, formalin or Kanamycin resulted in cells that could be no longer attached to these sites. Attachment of a Gfp(+) strain of Salm. Typhimurium to cell-wall material (CWM) was examined via flow cytometric analysis. Only live Salm. Typhimurium attached to the CWM. CONCLUSIONS: Salmonella serotype Typhimurium must be metabolically active to ensure attachment to vegetable tissues. Attachment preferentially occurs at the plant cell-wall junction and the cell-wall components found here, including pectate, may provide a receptor site for bacterial attachment. SIGNIFICANCE AND IMPACT OF THE STUDY: Further studies into individual plant cell-wall components may yield the specific bacterial receptor site in vegetable tissues. This information could in turn lead to the development of more targeted and effective decontamination protocols that block this site of attachment.
Authors:
E J Saggers; C R Waspe; M L Parker; K W Waldron; T F Brocklehurst
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-04-09
Journal Detail:
Title:  Journal of applied microbiology     Volume:  105     ISSN:  1365-2672     ISO Abbreviation:  J. Appl. Microbiol.     Publication Date:  2008 Nov 
Date Detail:
Created Date:  2009-01-16     Completed Date:  2010-02-22     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9706280     Medline TA:  J Appl Microbiol     Country:  England    
Other Details:
Languages:  eng     Pagination:  1239-45     Citation Subset:  IM    
Affiliation:
Institute of Food Research, Norwich Research Park, Norwich, UK. elizabeth.saggers@bbsrc.ac.uk
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MeSH Terms
Descriptor/Qualifier:
Bacterial Adhesion / physiology*
Cell Wall / microbiology*
Colony Count, Microbial
Ethanol
Flow Cytometry / methods
Food Microbiology
Microscopy, Electron, Scanning
Pectins / analysis
Salmonella typhimurium / growth & development,  physiology*
Solanum tuberosum / microbiology*,  ultrastructure
Temperature
Grant Support
ID/Acronym/Agency:
//Biotechnology and Biological Sciences Research Council
Chemical
Reg. No./Substance:
0/Pectins; 64-17-5/Ethanol; 9000-69-5/pectin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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