Document Detail


Salmonella exploits Arl8B-directed kinesin activity to promote endosome tubulation and cell-to-cell transfer.
MedLine Citation:
PMID:  21824248     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
The facultative intracellular pathogen Salmonella enterica serovar Typhimurium establishes a replicative niche, the Salmonella-containing vacuole (SCV), in host cells. Here we demonstrate that these bacteria exploit the function of Arl8B, an Arf family GTPase, during infection. Following infection, Arl8B localized to SCVs and to tubulated endosomes that extended along microtubules in the host cell cytoplasm. Arl8B(+) tubules partially colocalized with LAMP1 and SCAMP3. Formation of LAMP1(+) tubules (the Salmonella-induced filaments phenotype; SIFs) required Arl8B expression. SIFs formation is known to require the activity of kinesin-1. Here we find that Arl8B is required for kinesin-1 recruitment to SCVs. We have previously shown that SCVs undergo centrifugal movement to the cell periphery at 24 hours post infection and undergo cell-to-cell transfer to infect neighboring cells, and that both phenotypes require kinesin-1 activity. Here we demonstrate that Arl8B is required for migration of the SCV to the cell periphery 24 hours after infection and for cell-to-cell transfer of bacteria to neighboring cells. These results reveal a novel host factor co-opted by S. Typhimurium to manipulate the host endocytic pathway and to promote the spread of infection within a host.
Authors:
Natalia A Kaniuk; Veronica Canadien; Richard D Bagshaw; Malina Bakowski; Virginie Braun; Marija Landekic; Shuvadeep Mitra; Ju Huang; Won Do Heo; Tobias Meyer; Laurence Pelletier; Helene Andrews-Polymenis; Michael McClelland; Tony Pawson; Sergio Grinstein; John H Brumell
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-8-9
Journal Detail:
Title:  Cellular microbiology     Volume:  -     ISSN:  1462-5822     ISO Abbreviation:  -     Publication Date:  2011 Aug 
Date Detail:
Created Date:  2011-8-9     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100883691     Medline TA:  Cell Microbiol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
© 2011 Blackwell Publishing Ltd.
Affiliation:
Cell Biology Program, Hospital for Sick Children, Toronto, ON, Canada M5G 1X8 Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, ON, Canada, M5G 1X5 Department of Biological Sciences and KI for the BioCentury, KAIST, Daejeon 305-701, Korea Chemical and Systems Biology, Stanford University Medical School, Stanford, CA, USA 94305 Department of Microbial and Molecular Pathogenesis, Texas A&M Health Science Center Department of Pathology and Laboratory Medicine, University of California, Irvine, CA, 92697-4800Departments of Molecular Genetics, Biochemistry and Institute of Medical Science, University of Toronto, Toronto, ON, Canada, M5S 1A8.
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