| Salivary sIgA response in HIV-1 infection. | |
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MedLine Citation:
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PMID: 10360797 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Local and systemic production of total and HIV-1 specific IgA was determined in whole saliva and serum from 45 HIV-1-infected individuals and 15 healthy controls. The antigenic domains important for sIgA and IgG binding, respectively, was investigated with epitope mapping using synthetic peptides of HIV-1 proteins. Decreased levels of total sIgA in saliva were found among patients with low CD4+ cell counts in advanced stages of acquired immunodeficiency. HIV-1 specific IgA response, predominantly directed to the envelope proteins, was found in saliva and serum also at later stages of disease. Analyses using peptide enzyme-linked immunosorbent assays (ELISA) showed that the sIgA antibody response in saliva was mainly directed to the V4 region (aa 385-409) and a more C-terminal part of the V3-region (aa 325-344) compared with the IgG response, which predominantly was directed to a more central part of the V3 loop (aa 308-325). A similar picture was seen for immunoglobulins of the two isotypes derived from serum. We have in this study shown IgA epitope-specific immune response within HIV-1 gp160, indicating that antibodies of IgA isotype may recognize somewhat different antigenic domains compared to IgG antibodies. |
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Authors:
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P Skott; E Lucht; I Julander; J Dillner; E Björling |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of acquired immune deficiency syndromes (1999) Volume: 21 ISSN: 1525-4135 ISO Abbreviation: J. Acquir. Immune Defic. Syndr. Publication Date: 1999 Jun |
Date Detail:
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Created Date: 1999-06-16 Completed Date: 1999-06-16 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 100892005 Medline TA: J Acquir Immune Defic Syndr Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 73-80 Citation Subset: IM; X |
Affiliation:
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The Microbiology and Tumorbiology Center at Karolinska Institute, Stockholm, Sweden. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Acquired Immunodeficiency Syndrome
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classification,
complications,
immunology*,
pathology Adult Amino Acid Sequence Antibody Specificity* CD4 Lymphocyte Count Epitope Mapping Gene Products, env / immunology Gene Products, gag / immunology HIV Antibodies / analysis, blood, immunology* HIV-1 / immunology* Humans Immunity, Mucosal Immunoglobulin A, Secretory / analysis, blood, immunology* Immunoglobulin G / analysis, blood, immunology Molecular Sequence Data Mouth Diseases / complications, immunology, pathology Peptide Fragments / chemical synthesis, immunology Saliva / chemistry, immunology* |
| Chemical | |
Reg. No./Substance:
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0/Gene Products, env; 0/Gene Products, gag; 0/HIV Antibodies; 0/Immunoglobulin A, Secretory; 0/Immunoglobulin G; 0/Peptide Fragments |
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