| Salivary proteins of Russian wheat aphid (Hemiptera: Aphididae). | |
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MedLine Citation:
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PMID: 20146860 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Salivary secretions play critical roles in aphid-host plant interactions and are responsible for damage associated with aphid feeding. The objectives of this study were to evaluate aspects of salivation and the salivary constituents of Diuraphis noxia (Hemiptera: Aphididae). Salivary proteins were isolated and compared from three aphid probed diets: pure water, 15% sucrose, or amino acids (100 mM serine, 100 mM methionine, 100 mM aspartic acid, and 15% sucrose). After 6 h, more aphids settled on sucrose diet compared with other diets, but there were no significant differences in the number of stylet sheaths produced per aphid after 24 h. There were differences in the amount of soluble salivary protein (watery saliva), with the greatest amount secreted in sucrose diet, followed by amino acid diet and pure water, respectively. Protein constituents secreted into sucrose and amino acid diets were compared using gel electrophoresis using standardized amounts of protein. More protein bands and bands of greater intensity were visualized from probed sucrose diet compared with probed amino acid diet, indicating qualitative differences. Phosphatase was putatively identified from D. noxia saliva from a major protein band using gel electrophoresis and mass spectrophotometry. Alkaline phosphatase activity was confirmed in sucrose diet using enzymatic assays but was not detected in aphid probed water or amino acid diets. Other peptides in sucrose diet weakly but significantly showed similarities to putative dehydrogenase and RNA helicase expressed sequence tags identified from other aphids. The implications of these findings in aphid salivation and plant-insect interactions are discussed. |
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Authors:
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William R Cooper; Jack W Dillwith; Gary J Puterka |
Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Environmental entomology Volume: 39 ISSN: 1938-2936 ISO Abbreviation: Environ. Entomol. Publication Date: 2010 Feb |
Date Detail:
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Created Date: 2010-02-11 Completed Date: 2010-05-04 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 7502320 Medline TA: Environ Entomol Country: United States |
Other Details:
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Languages: eng Pagination: 223-31 Citation Subset: IM |
Affiliation:
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USDA-ARS, Wheat, Peanuts, and Other Field Crops, Stillwater, OK 74075-2714, USA. rodney.cooper@ars.usda.gov |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Alkaline Phosphatase
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analysis Animals Aphids / chemistry* Diet Electrophoresis, Polyacrylamide Gel Insect Proteins / analysis* Mass Spectrometry Saliva / chemistry* |
| Chemical | |
Reg. No./Substance:
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0/Insect Proteins; EC 3.1.3.1/Alkaline Phosphatase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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