Document Detail


Salivary protein/peptide profiling with SELDI-TOF-MS.
MedLine Citation:
PMID:  17435159     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In this study, large-scale profiling of salivary proteins and peptides ranging from 2 to 100 kDa was demonstrated using surface-enhanced laser desorption/ionization-time of flight-mass spectrometry (SELDI-TOF-MS). Results show that chip surface type and sample type critically affect the amount and composition of detected salivary proteins. Delayed processing time resulted in both increase and decrease of peak numbers consistent with proteolysis. SELDI-TOF-MS profiles also changed, depending on storage temperature, although sample processing by centrifugation and numbers of freeze-thaw cycles had a minimal impact. In conclusion, SELDI-TOF-MS offers a simple, rapid, high-throughput technique for profiling low-mass (<10 kDa) saliva proteins/peptides. We wish to use this technique to gain insight into the human saliva proteome composition and its changes over time in response to food consumption.
Authors:
Raymond Schipper; Arnoud Loof; Jolan de Groot; Lucien Harthoorn; Waander van Heerde; Eric Dransfield
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Annals of the New York Academy of Sciences     Volume:  1098     ISSN:  0077-8923     ISO Abbreviation:  Ann. N. Y. Acad. Sci.     Publication Date:  2007 Mar 
Date Detail:
Created Date:  2007-04-16     Completed Date:  2007-05-10     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  7506858     Medline TA:  Ann N Y Acad Sci     Country:  United States    
Other Details:
Languages:  eng     Pagination:  498-503     Citation Subset:  IM    
Affiliation:
Wageningen Centre for Food Sciences, TI Food and Nutrition, Laboratory of Food Chemistry, Wageningen University, the Netherlands. raymond.schipper@wur.nl
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MeSH Terms
Descriptor/Qualifier:
Eating
Female
Humans
Male
Peptides / analysis*,  metabolism
Saliva / chemistry*,  metabolism*
Salivary Proteins and Peptides / analysis*,  biosynthesis
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization*
Chemical
Reg. No./Substance:
0/Peptides; 0/Salivary Proteins and Peptides

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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