Document Detail

Salivary gland progenitor cells induced by duct ligation differentiate into hepatic and pancreatic lineages.
MedLine Citation:
PMID:  12829992     Owner:  NLM     Status:  MEDLINE    
Tissue damage can be assessed based on regenerative responses, including progenitor cell proliferation. In the salivary gland, tissue damage induced by ligation of main ducts leads to the disappearance of acinar cells and to marked proliferation of ductal cells. Reopening of the ducts leads to repopulation of acinar cells within 1 to 2 weeks, which suggests activation of tissue progenitor cells in a damaged state. Because submandibular glands derive from the endoderm and ectoderm, we investigated the possibility of the presence of endodermal progenitor cells. We cultured cells obtained from the ligated salivary gland and identified colonies of epithelium-like cells. We singled out and purified the cells by limited dilution, and one of the cells designated SGP-1 was used for further experiments. The SGP-1 expresses both alpha6beta1 integrin and cytoplasmic laminin. The hematopoietic stem cell marker CD34 and hepatic oval cell markers such as albumin, alpha-fetoprotein (AFP), and cytokeratin 19 are all negative. However, when SGP-1 cells were transplanted into the liver via the portal vein, these cells were integrated into hepatic trabecula and produced albumin. When SGP-1 cells formed clusters on type I collagen-coated dishes, they differentiated into endodermal lineage and 2 major types of clusters appeared: one contained cells positive for AFP and/or albumin (hepatic cluster) and the other positive for glucagon and/or insulin (pancreatic cluster). On laminin-coated dishes, SGP-1 selectively differentiated into hepatic-type cells. In conclusion, the multipotent progenitor cells isolated from the rat salivary gland have characteristics of tissue stem cells and can differentiate into cells of endodermal lineages.
Kenji Okumura; Kimitoshi Nakamura; Yuichiro Hisatomi; Koji Nagano; Yasuhiko Tanaka; Kunihiko Terada; Toshihiro Sugiyama; Kazuhiro Umeyama; Kozo Matsumoto; Tetsuro Yamamoto; Fumio Endo
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Hepatology (Baltimore, Md.)     Volume:  38     ISSN:  0270-9139     ISO Abbreviation:  Hepatology     Publication Date:  2003 Jul 
Date Detail:
Created Date:  2003-06-27     Completed Date:  2003-07-25     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8302946     Medline TA:  Hepatology     Country:  United States    
Other Details:
Languages:  eng     Pagination:  104-13     Citation Subset:  IM    
Department of Pediatrics, School of Medicine, Kumamoto University, Kumamoto, Japan.
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MeSH Terms
Cell Differentiation
Cell Lineage
Cell Separation
Cells, Cultured
Endoderm / cytology
Hepatocytes / cytology*
Pancreas / cytology*
Rats, Inbred LEC
Rats, Sprague-Dawley
Salivary Ducts
Stem Cell Transplantation
Stem Cells / cytology*
Submandibular Gland / cytology*

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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