Document Detail


Salivary gland protein repertoire from Aedes aegypti mosquitoes.
MedLine Citation:
PMID:  19877808     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Diseases caused by arthropod-borne viruses are a significant threat to the health of human and animal populations throughout the world. Better knowledge of the molecules synthesized in the salivary gland and saliva of hematophagous arthropods could be of use for improving the control of pathogen transmission. Recently, a sialome analysis of three Aedes aegypti mosquito colonies (PAEA, Rockefeller, and Formosus) carried out in our laboratory allowed us to identify 44 saliva proteins. Of these secreted proteins, none was exclusively expressed in one colony, suggesting that expression of salivary proteins is highly conserved across populations. In another study, we reported that some of these salivary proteins could be used as the genus-specific markers for travelers' exposure to mosquito vectors. Here, comparison of salivary gland protein profiles between these same three Ae. aegypti colonies was performed using the one-dimensional sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) difference gel electrophoresis method. As observed at the saliva level, no significant differences were detected between these three colonies. The salivary gland protein repertoire from the Ae. aegypti mosquito was analyzed using a proteomic approach. One hundred and twenty proteins were identified in these salivary glands representing the largest description of the Ae. aegypti salivary gland protein catalog. We succeeded in identifying 15 secreted proteins, some of which have already been reported as being involved in blood feeding. A comparison of the proteins identified between the salivary glands and the sialome is discussed.
Authors:
Lionel Almeras; Albin Fontaine; Maya Belghazi; Stéphanie Bourdon; Elodie Boucomont-Chapeaublanc; Eve Orlandi-Pradines; Meli Baragatti; Nicole Corre-Catelin; Paul Reiter; Bruno Pradines; Thierry Fusai; Christophe Rogier
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Vector borne and zoonotic diseases (Larchmont, N.Y.)     Volume:  10     ISSN:  1557-7759     ISO Abbreviation:  Vector Borne Zoonotic Dis.     Publication Date:  2010 May 
Date Detail:
Created Date:  2010-05-07     Completed Date:  2010-08-03     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100965525     Medline TA:  Vector Borne Zoonotic Dis     Country:  United States    
Other Details:
Languages:  eng     Pagination:  391-402     Citation Subset:  IM    
Affiliation:
Unité de Recherche en Biologie et Epidémiologie Parasitaires-UMR6236-IFR48, Institut de Médecine Tropicale du Service de Santé des Armées (IMTSSA), Marseille, France. almerasl@imtssa.fr
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MeSH Terms
Descriptor/Qualifier:
Aedes / metabolism*
Animals
Female
Gene Expression Profiling
Gene Expression Regulation / physiology
Insect Proteins / analysis,  metabolism*
Proteomics
Salivary Glands / metabolism
Salivary Proteins and Peptides / analysis,  metabolism*
Chemical
Reg. No./Substance:
0/Insect Proteins; 0/Salivary Proteins and Peptides

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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