Document Detail

Salicylhydroxamic acid inhibits delta6 desaturation in the microalga Porphyridium cruentum.
MedLine Citation:
PMID:  10446425     Owner:  NLM     Status:  MEDLINE    
Treatment of the microalga Porphyridium cruentum with salicylhydroxamic acid (SHAM) inhibited growth and affected fatty acid composition. At a relatively low concentration (40 microM) SHAM predominantly inhibits Delta6 desaturation. The effect of the inhibitor was most intense in phosphatidylcholine (PC) and phosphatidylethanolamine, in which the proportions of the downstream products of the Delta6 desaturase were reduced, whereas that of the substrate, 18:2, increased. As a result of the availability of 18:2, 18:3omega3, which under normal conditions is not observed, appeared predominantly in chloroplastic lipids. Pulse labeling with linoleic acid has shown that SHAM inhibits Delta6 desaturation almost immediately, suggesting an apparent inhibition of the activity of the desaturase, rather than its synthesis or that of its cofactors. Furthermore, the addition of gamma-linolenic acid to SHAM-inhibited cultures relieved the inhibition. Following exposure to the inhibitor, 18:3omega3 appeared first in chloroplastic glycolipids and only later in PC, indicating that the former are the substrates for the first dedicated step of the proposed omega3 pathway in this alga.
I Khozin-Goldberg; C Bigogno; Z Cohen
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biochimica et biophysica acta     Volume:  1439     ISSN:  0006-3002     ISO Abbreviation:  Biochim. Biophys. Acta     Publication Date:  1999 Aug 
Date Detail:
Created Date:  1999-09-17     Completed Date:  1999-09-17     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  0217513     Medline TA:  Biochim Biophys Acta     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  384-94     Citation Subset:  IM    
The Laboratory for Microalgal Biotechnology, Jacob Blaustein Institute for Desert Research, Sede-Boker Campus 84990, Israel.
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MeSH Terms
Algae, Red / drug effects,  enzymology*
Carbon Radioisotopes
Eicosapentaenoic Acid / metabolism
Fatty Acid Desaturases / antagonists & inhibitors*
Linoleoyl-CoA Desaturase
Salicylamides / pharmacology*
alpha-Linolenic Acid / pharmacology
Reg. No./Substance:
0/Carbon Radioisotopes; 0/Salicylamides; 1553-41-9/Eicosapentaenoic Acid; 463-40-1/alpha-Linolenic Acid; 89-73-6/salicylhydroxamic acid; EC 1.14.19.-/Fatty Acid Desaturases; EC Desaturase

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