Document Detail


Salacia oblonga root improves postprandial hyperlipidemia and hepatic steatosis in Zucker diabetic fatty rats: activation of PPAR-alpha.
MedLine Citation:
PMID:  15975614     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Salacia oblonga (SO) root is an Ayurvedic medicine with anti-diabetic and anti-obese properties. Peroxisome proliferator-activated receptor (PPAR)-alpha, a nuclear receptor, plays an important role in maintaining the homeostasis of lipid metabolism. Here, we demonstrate that chronic oral administration of the water extract from the root of SO to Zucker diabetic fatty (ZDF) rats, a genetic model of type 2 diabetes and obesity, lowered plasma triglyceride and total cholesterol (TC) levels, increased plasma high-density lipoprotein levels and reduced the liver contents of triglyceride, non-esterified fatty acids (NEFA) and the ratio of fatty droplets to total tissue. By contrast, the extract had no effect on plasma triglyceride and TC levels in fasted ZDF rats. After olive oil administration to ZDF the extract also inhibited the increase in plasma triglyceride levels. These results suggest that SO extract improves postprandial hyperlipidemia and hepatic steatosis in ZDF rats. Additionally, SO treatment enhanced hepatic expression of PPAR-alpha mRNA and protein, and carnitine palmitoyltransferase-1 and acyl-CoA oxidase mRNAs in ZDF rats. In vitro, SO extract and its main component mangiferin activated PPAR-alpha luciferase activity in human embryonic kidney 293 cells and lipoprotein lipase mRNA expression and enzyme activity in THP-1 differentiated macrophages; these effects were completely suppressed by a selective PPAR-alpha antagonist MK-886. The findings from both in vivo and in vitro suggest that SO extract functions as a PPAR-alpha activator, providing a potential mechanism for improvement of postprandial hyperlipidemia and hepatic steatosis in diabetes and obesity.
Authors:
Tom Hsun-Wei Huang; Gang Peng; George Qian Li; Johji Yamahara; Basil D Roufogalis; Yuhao Li
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Publication Detail:
Type:  Journal Article     Date:  2005-06-21
Journal Detail:
Title:  Toxicology and applied pharmacology     Volume:  210     ISSN:  0041-008X     ISO Abbreviation:  Toxicol. Appl. Pharmacol.     Publication Date:  2006 Feb 
Date Detail:
Created Date:  2006-01-23     Completed Date:  2006-03-16     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0416575     Medline TA:  Toxicol Appl Pharmacol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  225-35     Citation Subset:  IM    
Affiliation:
Herbal Medicines Research and Education Centre, Faculty of Pharmacy, The University of Sydney, NSW 2006, Australia.
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MeSH Terms
Descriptor/Qualifier:
Animals
Antilipemic Agents / pharmacology*
Cell Line
Cholesterol / blood
Fatty Liver / complications,  drug therapy*
Humans
Hyperlipidemias / complications,  drug therapy*
Lipoprotein Lipase / biosynthesis,  genetics
Luciferases / biosynthesis
Male
Medicine, Ayurvedic
Obesity / complications,  drug therapy
PPAR alpha / genetics,  metabolism*
Plant Extracts / pharmacology
Plant Roots
Postprandial Period
Rats
Rats, Zucker
Salacia*
Triglycerides / blood
Chemical
Reg. No./Substance:
0/Antilipemic Agents; 0/PPAR alpha; 0/Plant Extracts; 0/Triglycerides; 57-88-5/Cholesterol; EC 1.13.12.-/Luciferases; EC 3.1.1.34/Lipoprotein Lipase

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