Document Detail


Safety, efficacy and efficiency of laser-assisted IVF in subfertile mutant mouse strains.
MedLine Citation:
PMID:  23315689     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In the present report we studied the safety, efficacy and efficiency of using an infrared laser to facilitate IVF by assessing fertilization, development and birth rates after laser-zona drilling (LZD) in 30 subfertile genetically modified (GM) mouse lines. We determined that LZD increased the fertilization rate four to ten times that of regular IVF, thus facilitating the derivation of 26 of 30 (86.7%) GM mouse lines. Cryopreserved two-cell stage embryos derived by LZD-assisted IVF were recovered and developed to blastocysts in vitro at the same rate as frozen-thawed embryos derived by regular IVF. Surprisingly after surgical transfer to pseudopregnant recipients the birth rate of embryos derived by LZD-assisted IVF was significantly lower than that of embryos derived by regular IVF. However this result could be completely mitigated by the addition of 0.25 M sucrose to the culture medium during LZD which caused the oocyte to shrink in volume relative to the perivitelline space. By increasing the distance from the laser target site on the zona pellucida, we hypothesize that the hyperosmotic effect of sucrose reduced the potential for laser-induced cytotoxic thermal damage to the underlying oocytes. With appropriate preparation and cautious application, our results indicate that LZD-assisted IVF is a safe, efficacious and efficient assisted reproductive technology for deriving mutant mouse lines with male factor infertility and subfertility caused by sperm-zona penetration defects.
Authors:
Ming-Wen Li; Kristy L Kinchen; Jadine M Vallelunga; Diana L Young; Kaleb D K Wright; Lisa N Gorano; Katherine Wasson; K C Kent Lloyd
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2013-03-07
Journal Detail:
Title:  Reproduction (Cambridge, England)     Volume:  145     ISSN:  1741-7899     ISO Abbreviation:  Reproduction     Publication Date:  2013 Mar 
Date Detail:
Created Date:  2013-03-08     Completed Date:  2013-08-21     Revised Date:  2014-03-10    
Medline Journal Info:
Nlm Unique ID:  100966036     Medline TA:  Reproduction     Country:  England    
Other Details:
Languages:  eng     Pagination:  245-54     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Birth Rate
Blastocyst / physiology*
Cells, Cultured
Cryopreservation
Embryo Culture Techniques
Embryo Implantation
Embryo Transfer
Female
Fertilization in Vitro / adverse effects,  instrumentation*,  methods
Lasers*
Litter Size
Male
Mice
Mice, Inbred C57BL
Mice, Mutant Strains
Oocytes / drug effects,  physiology*
Pregnancy
Sucrose / pharmacology
Zona Pellucida / drug effects,  physiology*
Grant Support
ID/Acronym/Agency:
R24 RR018934/RR/NCRR NIH HHS; U42 OD011175/OD/NIH HHS; U42 OD012210/OD/NIH HHS; U42 RR024244/RR/NCRR NIH HHS; U42OD012210/OD/NIH HHS
Chemical
Reg. No./Substance:
57-50-1/Sucrose
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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