Document Detail

Safety, efficacy and efficiency of laser-assisted IVF in subfertile mutant mouse strains.
MedLine Citation:
PMID:  23315689     Owner:  NLM     Status:  MEDLINE    
In the present report we studied the safety, efficacy and efficiency of using an infrared laser to facilitate IVF by assessing fertilization, development and birth rates after laser-zona drilling (LZD) in 30 subfertile genetically modified (GM) mouse lines. We determined that LZD increased the fertilization rate four to ten times that of regular IVF, thus facilitating the derivation of 26 of 30 (86.7%) GM mouse lines. Cryopreserved two-cell stage embryos derived by LZD-assisted IVF were recovered and developed to blastocysts in vitro at the same rate as frozen-thawed embryos derived by regular IVF. Surprisingly after surgical transfer to pseudopregnant recipients the birth rate of embryos derived by LZD-assisted IVF was significantly lower than that of embryos derived by regular IVF. However this result could be completely mitigated by the addition of 0.25 M sucrose to the culture medium during LZD which caused the oocyte to shrink in volume relative to the perivitelline space. By increasing the distance from the laser target site on the zona pellucida, we hypothesize that the hyperosmotic effect of sucrose reduced the potential for laser-induced cytotoxic thermal damage to the underlying oocytes. With appropriate preparation and cautious application, our results indicate that LZD-assisted IVF is a safe, efficacious and efficient assisted reproductive technology for deriving mutant mouse lines with male factor infertility and subfertility caused by sperm-zona penetration defects.
Ming-Wen Li; Kristy L Kinchen; Jadine M Vallelunga; Diana L Young; Kaleb D K Wright; Lisa N Gorano; Katherine Wasson; K C Kent Lloyd
Related Documents :
24616249 - Senescence-associated β-galactosidase activity marks the visceral endoderm of mouse em...
24691849 - Yeast diversity associated with invasive dendroctonus valens killing pinus tabuliformis...
23818829 - High efficiency secondary somatic embryogenesis in hovenia dulcis thunb. through solid ...
22976279 - Nucleoli from two-cell embryos support the development of enucleolated germinal vesicle...
24031199 - Detection of salmonella sp in chicken cuts using immunomagnetic separation.
23442979 - Replacement of glutamine with the dipeptide derivative alanyl-glutamine enhances in vit...
8495729 - Cell-penetrating inhibitors of calpain block both membrane fusion and filamin cleavage ...
15710189 - Angiotensin ii reverses the inhibitory action produced by theca cells on bovine oocyte ...
16393449 - Resilient individuals improve evolutionary search.
Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2013-03-07
Journal Detail:
Title:  Reproduction (Cambridge, England)     Volume:  145     ISSN:  1741-7899     ISO Abbreviation:  Reproduction     Publication Date:  2013 Mar 
Date Detail:
Created Date:  2013-03-08     Completed Date:  2013-08-21     Revised Date:  2014-03-10    
Medline Journal Info:
Nlm Unique ID:  100966036     Medline TA:  Reproduction     Country:  England    
Other Details:
Languages:  eng     Pagination:  245-54     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Birth Rate
Blastocyst / physiology*
Cells, Cultured
Embryo Culture Techniques
Embryo Implantation
Embryo Transfer
Fertilization in Vitro / adverse effects,  instrumentation*,  methods
Litter Size
Mice, Inbred C57BL
Mice, Mutant Strains
Oocytes / drug effects,  physiology*
Sucrose / pharmacology
Zona Pellucida / drug effects,  physiology*
Grant Support
R24 RR018934/RR/NCRR NIH HHS; U42 OD011175/OD/NIH HHS; U42 OD012210/OD/NIH HHS; U42 RR024244/RR/NCRR NIH HHS; U42OD012210/OD/NIH HHS
Reg. No./Substance:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Age-dependent inhibin B concentration in relation to FSH and semen sample qualities: a study in 2448...
Next Document:  [The relationship between DSM-IV axis II and the anaclitic and introjective personality dimensions].