Document Detail


The Saccharomyces cerevisiae spindle pole body is a dynamic structure.
MedLine Citation:
PMID:  12925780     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
During spindle pole body (SPB) duplication, the new SPB is assembled at a distinct site adjacent to the old SPB. Using quantitative fluorescence methods, we studied the assembly and dynamics of the core structural SPB component Spc110p. The SPB core exhibits both exchange and growth in a cell cycle-dependent manner. During G1/S phase, the old SPB exchanges approximately 50% of old Spc110p for new Spc110p. In G2 little Spc110p is exchangeable. Thus, Spc110p is dynamic during G1/S and becomes stable during G2. The SPB incorporates additional Spc110p in late G2 and M phases; this growth is followed by reduction in the next G1. Spc110p addition to the SPBs (growth) also occurs in response to G2 and mitotic arrests but not during a G1 arrest. Our results reveal several dynamic features of the SPB core: cell cycle-dependent growth and reduction, growth in response to cell cycle arrests, and exchange of Spc110p during SPB duplication. Moreover, rather than being considered a conservative or dispersive process, the assembly of Spc110p into the SPB is more readily considered in terms of growth and exchange.
Authors:
Tennessee J Yoder; Chad G Pearson; Kerry Bloom; Trisha N Davis
Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.     Date:  2003-05-03
Journal Detail:
Title:  Molecular biology of the cell     Volume:  14     ISSN:  1059-1524     ISO Abbreviation:  Mol. Biol. Cell     Publication Date:  2003 Aug 
Date Detail:
Created Date:  2003-08-19     Completed Date:  2004-05-25     Revised Date:  2014-09-16    
Medline Journal Info:
Nlm Unique ID:  9201390     Medline TA:  Mol Biol Cell     Country:  United States    
Other Details:
Languages:  eng     Pagination:  3494-505     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Cell Cycle
Cytoskeletal Proteins
G1 Phase
G2 Phase
Mitosis
Nuclear Proteins / metabolism*
Saccharomyces cerevisiae / cytology,  metabolism*
Saccharomyces cerevisiae Proteins / metabolism*
Spindle Apparatus / metabolism*
Grant Support
ID/Acronym/Agency:
GM-32238/GM/NIGMS NIH HHS; GM-40506/GM/NIGMS NIH HHS; R01 GM032238/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/Cytoskeletal Proteins; 0/Nuclear Proteins; 0/SPC110 protein, S cerevisiae; 0/Saccharomyces cerevisiae Proteins
Comments/Corrections

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