Document Detail


Saccharomyces cerevisiae gene expression changes during rotating wall vessel suspension culture.
MedLine Citation:
PMID:  12391061     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
This study utilizes Saccharomyces cerevisiae to study genetic responses to suspension culture. The suspension culture system used in this study is the high-aspect-ratio vessel, one type of the rotating wall vessel, that provides a high rate of gas exchange necessary for rapidly dividing cells. Cells were grown in the high-aspect-ratio vessel, and DNA microarray and metabolic analyses were used to determine the resulting changes in yeast gene expression. A significant number of genes were found to be up- or downregulated by at least twofold as a result of rotational growth. By using Gibbs promoter alignment, clusters of genes were examined for promoter elements mediating these genetic changes. Candidate binding motifs similar to the Rap1p binding site and the stress-responsive element were identified in the promoter regions of differentially regulated genes. This study shows that, as in higher order organisms, S. cerevisiae changes gene expression in response to rotational culture and also provides clues for investigations into the signaling pathways involved in gravitational response.
Authors:
Kelly Johanson; Patricia L Allen; Fawn Lewis; Luis A Cubano; Linda E Hyman; Timothy G Hammond
Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2002-08-16
Journal Detail:
Title:  Journal of applied physiology (Bethesda, Md. : 1985)     Volume:  93     ISSN:  8750-7587     ISO Abbreviation:  J. Appl. Physiol.     Publication Date:  2002 Dec 
Date Detail:
Created Date:  2002-11-15     Completed Date:  2003-05-12     Revised Date:  2013-09-26    
Medline Journal Info:
Nlm Unique ID:  8502536     Medline TA:  J Appl Physiol (1985)     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2171-80     Citation Subset:  IM; S    
Affiliation:
Department of Biochemistry, Center for BioEnvironmental Research, Tulane University Health Sciences Center, New Orleans, Louisiana 70112, USA.
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MeSH Terms
Descriptor/Qualifier:
Blotting, Northern
Cluster Analysis
Gene Expression Regulation, Fungal*
Gravitation*
Oligonucleotide Array Sequence Analysis
Promoter Regions, Genetic / genetics
Rotation
Saccharomyces cerevisiae / growth & development,  physiology*
Investigator
Investigator/Affiliation:
T G Hammond / VA Med Ctr, New Orleans, LA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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