Document Detail


Saccharomyces cerevisiae colony growth and ageing: biphasic growth accompanied by changes in gene expression.
MedLine Citation:
PMID:  10487919     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Although colony growth and morphology are central tools in yeast genetics, little is known about the cell physiology and how it changes during the colony growth and ageing. Here we show that the growth of a well-separated Saccharomyces cerevisiae colony is biphasic; a rapid growth phase is followed by a sharp transition to a slower growth phase. In the first growth phase ( approximately 24 cell divisions) most, if not all, cells divide at a rate similar to that in liquid medium and exhibit morphological, biochemical and genetic characteristics of cells engaged in the cell cycle. During the second growth phase, cells in the centre of a colony gradually enter stationary phase, so that later in this phase the growth occurs predominantly at the periphery. Unlike the biphasic growth in rich liquid media containing a fermentable carbon source, in which the first growth is fueled by fermentation and the second by aerobic metabolism, the two phases of the colony growth can be fueled either exclusively by fermentation or exclusively by aerobic metabolism. We also describe a novel technique for in situ estimation of the transcriptional status in the colony cells, which was used to monitor transcription dynamics during the colony development. Using this technique and standard methods to determine mRNA levels, we show that the transition between the first and second growth phases is accompanied by a global change in the pattern of transcription: transcription of most genes is repressed while that of some genes is induced.
Authors:
J R Meunier; M Choder
Related Documents :
12122059 - Intrinsic program for migration of cerebellar granule cells in vitro.
1304669 - Effects of hyperoxia on human endothelial cell proliferation and morphology in vitro.
24224529 - Quantitative proteomics reveals significant changes in cell shape and an energy shift a...
7237289 - K+, na+, and mg2+ content and permeability of methanospirillum hungatei and methanobact...
18851059 - Coherence, collective rhythm, and phase difference distribution in populations of stoch...
4599119 - Separation of erythromycin-resistant and -susceptible subpopulations of escherichia col...
22416289 - The tasks of amacrine cells.
8671339 - Stromal cell interaction and relevance to predecidual events and menstruation.
16278919 - Simultaneous determination of glutathione and reactive oxygen species in individual cel...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Yeast (Chichester, England)     Volume:  15     ISSN:  0749-503X     ISO Abbreviation:  Yeast     Publication Date:  1999 Sep 
Date Detail:
Created Date:  1999-12-14     Completed Date:  1999-12-14     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8607637     Medline TA:  Yeast     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  1159-69     Citation Subset:  IM    
Copyright Information:
Copyright 1999 John Wiley & Sons, Ltd.
Affiliation:
Department of Cell Biology, Weizmann Institute of Science, Rehovot 76100, Israel.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Adenosine Triphosphate / metabolism
Aerobiosis
Cell Cycle*
Cell Division
Fermentation
Gene Expression Regulation, Fungal*
Genes, Fungal / genetics
Genes, Reporter / genetics
Kinetics
Mitosis
RNA, Fungal / analysis,  genetics
RNA, Messenger / analysis,  genetics
Saccharomyces cerevisiae / cytology,  genetics*,  growth & development*,  metabolism
Transcription, Genetic / genetics
Chemical
Reg. No./Substance:
0/RNA, Fungal; 0/RNA, Messenger; 56-65-5/Adenosine Triphosphate

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Sensorimotor polyneuropathy associated with chronic lymphocytic leukemia, IgM antigangliosides antib...
Next Document:  A mutant for the yeast scERV1 gene displays a new defect in mitochondrial morphology and distributio...