Document Detail

Saccharomyces cerevisiae colony growth and ageing: biphasic growth accompanied by changes in gene expression.
MedLine Citation:
PMID:  10487919     Owner:  NLM     Status:  MEDLINE    
Although colony growth and morphology are central tools in yeast genetics, little is known about the cell physiology and how it changes during the colony growth and ageing. Here we show that the growth of a well-separated Saccharomyces cerevisiae colony is biphasic; a rapid growth phase is followed by a sharp transition to a slower growth phase. In the first growth phase ( approximately 24 cell divisions) most, if not all, cells divide at a rate similar to that in liquid medium and exhibit morphological, biochemical and genetic characteristics of cells engaged in the cell cycle. During the second growth phase, cells in the centre of a colony gradually enter stationary phase, so that later in this phase the growth occurs predominantly at the periphery. Unlike the biphasic growth in rich liquid media containing a fermentable carbon source, in which the first growth is fueled by fermentation and the second by aerobic metabolism, the two phases of the colony growth can be fueled either exclusively by fermentation or exclusively by aerobic metabolism. We also describe a novel technique for in situ estimation of the transcriptional status in the colony cells, which was used to monitor transcription dynamics during the colony development. Using this technique and standard methods to determine mRNA levels, we show that the transition between the first and second growth phases is accompanied by a global change in the pattern of transcription: transcription of most genes is repressed while that of some genes is induced.
J R Meunier; M Choder
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Yeast (Chichester, England)     Volume:  15     ISSN:  0749-503X     ISO Abbreviation:  Yeast     Publication Date:  1999 Sep 
Date Detail:
Created Date:  1999-12-14     Completed Date:  1999-12-14     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8607637     Medline TA:  Yeast     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  1159-69     Citation Subset:  IM    
Copyright Information:
Copyright 1999 John Wiley & Sons, Ltd.
Department of Cell Biology, Weizmann Institute of Science, Rehovot 76100, Israel.
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MeSH Terms
Adenosine Triphosphate / metabolism
Cell Cycle*
Cell Division
Gene Expression Regulation, Fungal*
Genes, Fungal / genetics
Genes, Reporter / genetics
RNA, Fungal / analysis,  genetics
RNA, Messenger / analysis,  genetics
Saccharomyces cerevisiae / cytology,  genetics*,  growth & development*,  metabolism
Transcription, Genetic / genetics
Reg. No./Substance:
0/RNA, Fungal; 0/RNA, Messenger; 56-65-5/Adenosine Triphosphate

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