| The Saccharomyces cerevisiae checkpoint gene BUB1 encodes a novel protein kinase. | |
| | |
MedLine Citation:
|
PMID: 7969164 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Normal cell multiplication requires that the events of mitosis occur in a carefully ordered fashion. Cells employ checkpoints to prevent cycle progression until some prerequisite step has been completed. To explore the mechanisms of checkpoint enforcement, we previously screened for mutants of Saccharomyces cerevisiae which are unable to recover from a transient treatment with a benzimidazole-related microtubule inhibitor because they fail to inhibit subsequent cell cycle steps. Two of the identified genes, BUB2 and BUB3, have been cloned and described (M. A. Hoyt, L. Totis, and B. T. Roberts, Cell 66:507-517, 1991). Here we present the characterization of the BUB1 gene and its product. Genetic evidence was obtained suggesting that Bub1 and Bub3 are mutually dependent for function, and immunoprecipitation experiments demonstrated a physical association between the two. Sequence analysis of BUB1 revealed a domain with similarity to protein kinases. In vitro experiments confirmed that Bub1 possesses kinase activity; Bub1 was able to autophosphorylate and to catalyze phosphorylation of Bub3. In addition, overproduced Bub1 was found to localize to the cell nucleus. |
| | |
Authors:
|
B T Roberts; K A Farr; M A Hoyt |
Related Documents
:
|
11089874 - Cell cycle activation by plant parasitic nematodes. 17976384 - Signal-induced functions of the transcription factor tfii-i. 19147494 - Ksr1 is required for cell cycle reinitiation following dna damage. 21116964 - Evaluation of cytotoxicity of nanoparticulate materials in porcine kidney cells and hum... 7014864 - Embryonic and uterine factors in delayed implantation in rodents. 15041224 - The occurrence of chromosome segregational defects is an intrinsic and heritable proper... |
Publication Detail:
|
Type: Comparative Study; Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
|
Title: Molecular and cellular biology Volume: 14 ISSN: 0270-7306 ISO Abbreviation: Mol. Cell. Biol. Publication Date: 1994 Dec |
Date Detail:
|
Created Date: 1994-12-20 Completed Date: 1994-12-20 Revised Date: 2009-11-19 |
Medline Journal Info:
|
Nlm Unique ID: 8109087 Medline TA: Mol Cell Biol Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 8282-91 Citation Subset: IM |
Affiliation:
|
Department of Biology, Johns Hopkins University, Baltimore, Maryland 21218-2685. |
| Data Bank Information | |
Bank Name/Acc. No.:
|
GENBANK/L32027 |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Amino Acid Sequence Base Sequence Cell Cycle* Cell Cycle Proteins* Cloning, Molecular Fungal Proteins / genetics, metabolism* Genes, Fungal* Molecular Sequence Data Mutagenesis, Insertional Nuclear Proteins / metabolism Protein Binding Protein Kinases / genetics*, metabolism Protein-Serine-Threonine Kinases Restriction Mapping Saccharomyces cerevisiae / genetics* Saccharomyces cerevisiae Proteins* Sequence Alignment Sequence Homology, Amino Acid Structure-Activity Relationship |
| Grant Support | |
ID/Acronym/Agency:
|
GM49363/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/BUB3 protein, S cerevisiae; 0/Cell Cycle Proteins; 0/Fungal Proteins; 0/Nuclear Proteins; 0/Saccharomyces cerevisiae Proteins; EC 2.7.-/Protein Kinases; EC 2.7.11.1/Bub1 spindle checkpoint protein; EC 2.7.11.1/Protein-Serine-Threonine Kinases |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Type I adenylyl cyclase functions as a coincidence detector for control of cyclic AMP response eleme...
Next Document: The Ikaros gene encodes a family of functionally diverse zinc finger DNA-binding proteins.