Document Detail


Activation of two different resistance mechanisms in Saccharomyces cerevisiae upon exposure to octanoic and decanoic acids.
MedLine Citation:
PMID:  20851956     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Medium-chain fatty acids (octanoic and decanoic acids) are well known as fermentation inhibitors. During must fermentation, the toxicity of these fatty acids is enhanced by ethanol and low pH, which favors their entrance in the cell, resulting in a decrease of internal pH. We present here the characterization of the mechanisms involved in the establishment of the resistance to these fatty acids. The analysis of the transcriptome response to the exposure to octanoic and decanoic acids revealed that two partially overlapping mechanisms are activated; both responses share many genes with an oxidative stress response, but some key genes were activated differentially. The transcriptome response to octanoic acid stress can be described mainly as a weak acid response, and it involves Pdr12p as the main transporter. The phenotypic analysis of knocked-out strains confirmed the role of the Pdr12p transporter under the control of WAR1 but also revealed the involvement of the Tpo1p major facilitator superfamily proteins (MFS) transporter in octanoic acid expulsion. In contrast, the resistance to decanoic acid is composite. It also involves the transporter Tpo1p and includes the activation of several genes of the beta-oxidation pathway and ethyl ester synthesis. Indeed, the induction of FAA1 and EEB1, coding for a long-chain fatty acyl coenzyme A synthetase and an alcohol acyltransferase, respectively, suggests a detoxification pathway through the production of decanoate ethyl ester. These results are confirmed by the sensitivity of strains bearing deletions for the transcription factors encoded by PDR1, STB5, OAF1, and PIP2 genes.
Authors:
J L Legras; C Erny; C Le Jeune; M Lollier; Y Adolphe; C Demuyter; P Delobel; B Blondin; F Karst
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-09-17
Journal Detail:
Title:  Applied and environmental microbiology     Volume:  76     ISSN:  1098-5336     ISO Abbreviation:  Appl. Environ. Microbiol.     Publication Date:  2010 Nov 
Date Detail:
Created Date:  2010-11-08     Completed Date:  2011-02-22     Revised Date:  2011-07-28    
Medline Journal Info:
Nlm Unique ID:  7605801     Medline TA:  Appl Environ Microbiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  7526-35     Citation Subset:  IM    
Affiliation:
UMR 1083 Sciences pour l'Oenologie INRA, SupAgro, Montpellier cedex 1, France. jean-luc.legras@supagro.inra.fr
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MeSH Terms
Descriptor/Qualifier:
Antifungal Agents / toxicity*
Decanoic Acids / toxicity*
Drug Resistance, Fungal*
Gene Expression Profiling
Gene Expression Regulation, Fungal / drug effects
Octanoic Acids / toxicity*
Saccharomyces cerevisiae / drug effects*
Saccharomyces cerevisiae Proteins / metabolism
Stress, Physiological
Chemical
Reg. No./Substance:
0/Antifungal Agents; 0/Decanoic Acids; 0/Octanoic Acids; 0/Saccharomyces cerevisiae Proteins
Comments/Corrections

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