Document Detail


Saccharomyces cerevisiae HOC1, a suppressor of pkc1, encodes a putative glycosyltransferase.
MedLine Citation:
PMID:  9055074     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The Saccharomyces cerevisiae gene PKC1 encodes a protein kinase C isozyme that regulates cell wall synthesis. Here we describe the characterization of HOC1, a gene identified by its ability to suppress the cell lysis phenotype of pkc1-371 cells. The HOC1 gene (Homologous to OCH1) is predicted to encode a type II integral membrane protein that strongly resembles Och 1p, an alpha-1,6-mannosyltransferase. Immunofluorescence studies localized Hoc1p to the Golgi apparatus. While overexpression of HOC1 rescued the pkc1-371 temperature-sensitive cell lysis phenotype, disruption of HOC1 lowered the restrictive temperature of the pkc1-371 allele. Disruption of HOC1 also resulted in hypersensitivity to Calcofluor White and hygromycin B, phenotypes characteristic of defects in cell wall integrity and protein glycosylation, respectively. The function of HOC1 appears to be distinct from that of OCH1. Taken together, these results suggest that HOC1 encodes a Golgi-localized putative mannosyltransferase required for the proper construction of the cell wall.
Authors:
A M Neiman; V Mhaiskar; V Manus; F Galibert; N Dean
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Genetics     Volume:  145     ISSN:  0016-6731     ISO Abbreviation:  Genetics     Publication Date:  1997 Mar 
Date Detail:
Created Date:  1997-05-29     Completed Date:  1997-05-29     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0374636     Medline TA:  Genetics     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  637-45     Citation Subset:  IM    
Affiliation:
Department of Biochemistry and Cell Biology, State University of New York, Stony Brook 11794-5215, USA.
Data Bank Information
Bank Name/Acc. No.:
GENBANK/U62942
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Base Sequence
Benzenesulfonates / pharmacology
Cloning, Molecular
DNA, Recombinant
Fluorescent Antibody Technique, Indirect
Genes, Suppressor*
Glycosyltransferases / genetics*
Golgi Apparatus / metabolism
Hygromycin B / pharmacology
Membrane Proteins / genetics*
Molecular Sequence Data
Protein Kinase C / antagonists & inhibitors*,  genetics
Saccharomyces cerevisiae / enzymology,  genetics*
Sequence Homology, Amino Acid
Temperature
Grant Support
ID/Acronym/Agency:
GM-28220/GM/NIGMS NIH HHS; GM-48467/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/Benzenesulfonates; 0/DNA, Recombinant; 0/Membrane Proteins; 31282-04-9/Hygromycin B; 4404-43-7/C.I. Fluorescent Brightening Agent 28; EC 2.4.-/Glycosyltransferases; EC 2.4.-/HOC1 protein; EC 2.7.11.13/Protein Kinase C
Comments/Corrections

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