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Sabouraud dextrose agar for the diagnosis of fungal keratitis.
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MedLine Citation:
PMID:  20952848     Owner:  NLM     Status:  MEDLINE    
Viroj Wiwanitkit
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Publication Detail:
Type:  Comment; Letter    
Journal Detail:
Title:  Indian journal of ophthalmology     Volume:  58     ISSN:  1998-3689     ISO Abbreviation:  Indian J Ophthalmol     Publication Date:    2010 Nov-Dec
Date Detail:
Created Date:  2010-10-18     Completed Date:  2011-02-01     Revised Date:  2013-07-03    
Medline Journal Info:
Nlm Unique ID:  0405376     Medline TA:  Indian J Ophthalmol     Country:  India    
Other Details:
Languages:  eng     Pagination:  549-50; author reply 550     Citation Subset:  IM    
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MeSH Terms
Cornea / microbiology*
Culture Media*
Eye Infections, Fungal / diagnosis*
Keratitis / diagnosis*
Reg. No./Substance:
0/Culture Media
Comment On:
Indian J Ophthalmol. 2010 Jul-Aug;58(4):281-6   [PMID:  20534916 ]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Full Text
Journal Information
Journal ID (nlm-ta): Indian J Ophthalmol
Journal ID (publisher-id): IJO
ISSN: 0301-4738
ISSN: 1998-3689
Publisher: Medknow Publications, India
Article Information
© Indian Journal of Ophthalmology
Print publication date: Season: Nov–Dec Year: 2010
Volume: 58 Issue: 6
First Page: 549 Last Page: 550
Publisher Id: IJO-58-549c
PubMed Id: 20952848
DOI: 10.4103/0301-4738.71684

Sabouraud dextrose agar for the diagnosis of fungal keratitis
Viroj WiwanitkitAF0001
Wiwanitkit House, Bangkhae, Bangkok – 10160, Thailand
Correspondence: Correspondence to: Prof. Viroj Wiwanitkit, Wiwanitkit House, Bangkhae, Bangkok – 10160, Thailand. E-mail:

Dear Editor,

I read the recent publication on diagnosis of fungal keratitis with great interest.[1] Das et al. concluded that “This study recommends use of blood agar or chocolate agar for diagnosis of both bacterial and fungal keratitis in situations with limited resources.”[1] Omitting sabouraud dextrose agar (SDA) seems to be a challenge. In microbiology, SDA is used as a standard for fungus study.[2] The use of few samples in this study should be carefully considered. In addition, some fungi, especially for Histoplasma spp. cannot be successfully cultured via blood or chocolate agars.[34]

1. Das S,Sharma S,Kar S,Sahu SK,Samal B,Mallick A. Is inclusion of Sabouraud dextrose agar essential for the laboratory diagnosis of fungal keratitis?Indian J OphthalmolYear: 201058281620534916
2. Odds FC. Sabouraud(’s) agarJ Med Vet MycolYear: 19912935591815027
3. Procop GW,Cockerill FR 3rd,Vetter EA,Harmsen WS,Hughes JG,Roberts GD. Performance of five agar media for recovery of fungi from isolator blood culturesJ Clin MicrobiolYear: 2000383827911015411
4. Roberts GD,Washington JA 2nd. Detection of fungi in blood culturesJ Clin MicrobiolYear: 19751309101176606

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