| SYNTHESIS OF A PRECURSOR TRIPEPTIDE Z-Asp-Val-Tyr-OH OF THYMOPENTIN BY CHEMO-ENZYMATIC METHOD. | |
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MedLine Citation:
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PMID: 23030464 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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The precursor tripeptide of thymopentin was synthesized by a combination of chemical and enzymatic methods. First, Val-Tyr-OH dipeptide was synthesized by a novel chemical method in two steps involving preparation of NCA-Val. Second, the linkage of the third amino acid Z-Asp-OMe to Val-Tyr-OH was completed by an enzymatic method under kinetic control. An industrial alkaline protease alcalase was used in water-organic cosolvent systems. The synthesis reaction conditions were optimized by examining the effects of several factors including organic solvents, water content, temperature, pH, and reaction time on the yield of Z-Asp-Val-Tyr-OH. The optimum condition is of pH 10.0, 35°C, acetonitrile/Na(2)CO(3)-NaHCO(3) buffer system (85:15, v/v), and reaction time of 2.5 hr, which achieves tripeptide yield of more than 70%. |
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Authors:
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Kun Zheng; Ruiyan Zhan; Yang Hong; Jing Li; Wei Shi; Shijun Li |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Preparative biochemistry & biotechnology Volume: 42 ISSN: 1532-2297 ISO Abbreviation: Prep. Biochem. Biotechnol. Publication Date: 2012 Nov |
Date Detail:
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Created Date: 2012-10-03 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9607037 Medline TA: Prep Biochem Biotechnol Country: England |
Other Details:
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Languages: eng Pagination: 520-34 Citation Subset: IM |
Affiliation:
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a Key Laboratory for Molecular Enzymology and Engineering, Ministry of Education, Jilin University , Changchun , P.R. China. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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