Document Detail


SYBR green real-time PCR used to detect celery in food.
MedLine Citation:
PMID:  21140666     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Celery was found to provoke human allergenic response in some countries. Labeling of celery ingredients was required by the European Union, and the threshold set at 10 mg/kg (0.001%). In our study, a celery mannitol transporter (Mat3) gene-based detection method was established by means of SYBR Green real-time PCR technique. No cross-reactivity was found between celery and the other food materials. Absolute detection limit (LODa), relative detection limit (LODr), and practical detection limit (LODp) of the method were determined through experiments on pure celery DNA, DNA mix, and spiked food samples. The method was able to detect 0.001% raw food sample and 0.01% heated food sample. The utility of the method was confirmed by the investigation of 13 commercial foods.
Authors:
Yajun Wu; Ying Chen; Bin Wang; Yunhua Gao; Liqun Bai; Haiyan Wang
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of AOAC International     Volume:  93     ISSN:  1060-3271     ISO Abbreviation:  J AOAC Int     Publication Date:    2010 Sep-Oct
Date Detail:
Created Date:  2010-12-09     Completed Date:  2010-12-21     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9215446     Medline TA:  J AOAC Int     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1530-6     Citation Subset:  IM    
Affiliation:
Chinese Academy of Inspection and Quarantine, No. 3, Gaobeidian North St, Chaoyang District, Beijing, 100123, People's Republic of China.
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MeSH Terms
Descriptor/Qualifier:
Apium graveolens / genetics*
Base Sequence
DNA, Plant / analysis*
Food Analysis / methods*
Limit of Detection
Molecular Sequence Data
Organic Chemicals
Polymerase Chain Reaction / methods*
Chemical
Reg. No./Substance:
0/DNA, Plant; 0/Organic Chemicals; 163795-75-3/SYBR Green I

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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