Document Detail

SV40-transformed cells with temperature-dependent serum requirements.
MedLine Citation:
PMID:  164289     Owner:  NLM     Status:  MEDLINE    
We have isolated temperature-sensitive SV40-transformed 3T3 cells which are unable to grow in low or depleted serum at the nonpermissive temperature. At 39 degrees C, these cells do not grow in 1 percent serum, but they grow if the serum concentration is raised to 10 percent. At 32 degrees they grow in both serum concentrations. This phenotype seems to be due to a cellular mutation, as the virus rescued from these cells is wild-type. We tested whether other characteristics of transformed cells were expressed in a temperature sensitive way. While high saturation density is ts in these cells, other parameters of transformation are expressed at both temperatures. In addition, when these cells are incubated in low serum at 39 degrees C, they keep synthesizing DNA and lose viability very fast, while under the same conditions normal 3T3 cells remain viable for long times and are unable to initiate DNA synthesis. These cells therefore do not appear to revert to a normal phenotype at the high temperature, and they are more likely to represent transformed cell variants with a temperature-dependent serum requirement.
D Toniolo; C Basilico
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Cell     Volume:  4     ISSN:  0092-8674     ISO Abbreviation:  Cell     Publication Date:  1975 Mar 
Date Detail:
Created Date:  1975-06-27     Completed Date:  1975-06-27     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  0413066     Medline TA:  Cell     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  255-62     Citation Subset:  IM    
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MeSH Terms
Cell Aggregation
Cell Division
Cell Survival
Cell Transformation, Neoplastic*
Cells, Cultured
Culture Media
Cyclic AMP / metabolism
Fluorescent Antibody Technique
Simian virus 40*
Time Factors
Reg. No./Substance:
0/Culture Media; 60-92-4/Cyclic AMP

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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