| SV40 DNA amplification and reintegration in surviving hamster cells after 60Co gamma-irradiation. | |
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MedLine Citation:
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PMID: 1976719 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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SV40-transformed Chinese hamster embryo cells were exposed to 60Co gamma-irradiation and the fate of the integrated SV40 sequences was pursued over a period of 20 days following radiation exposure. As shown by colony hybridization, integrated SV40 sequences were amplified in surviving and non-surviving cells. At later times, however, clonal sublines of surviving cells grown for 20-30 cell generations after irradiation had lost most of their amplified SV40 copies but showed altered restriction fragment patterns indicating reintegration of SV40 sequences at new sites of the hamster genome. This suggests that 60Co gamma-irradiation can generate mutations by inducing over-replication of chromosome segments that are then substrates of enzymatic rearrangements. |
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Authors:
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C Lücke-Huhle; M Pech; P Herrlich |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: International journal of radiation biology Volume: 58 ISSN: 0955-3002 ISO Abbreviation: Int. J. Radiat. Biol. Publication Date: 1990 Oct |
Date Detail:
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Created Date: 1990-11-16 Completed Date: 1990-11-16 Revised Date: 2003-11-14 |
Medline Journal Info:
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Nlm Unique ID: 8809243 Medline TA: Int J Radiat Biol Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 577-88 Citation Subset: IM; S |
Affiliation:
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Kerforschungszentrum Karlsruhe, Institut für Genetik und für Toxikologie von Spaltstoffen, FRG. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Line, Transformed Cell Survival / radiation effects* Cobalt Radioisotopes DNA, Viral / genetics* Gamma Rays Gene Amplification* Simian virus 40 / genetics* |
| Chemical | |
Reg. No./Substance:
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0/Cobalt Radioisotopes; 0/DNA, Viral |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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