Document Detail


SUMOylation of Pax7 is essential for neural crest and muscle development.
MedLine Citation:
PMID:  23247248     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Regulatory transcription factors of the Pax family play fundamental roles in the function of multipotent cells during vertebrate development, post-natal regeneration, and cancer. Pax7 and its homologue Pax3 are important players in neural crest and muscle development. Both genes are coexpressed in various tissues and are thought to provide similar, but not identical, functions. The mechanisms that allow specific regulation of Pax7 remain largely unknown. Here, we report for the first time that Pax7 is regulated by SUMOylation. We identify the interaction of Pax7 with Ubc9, the SUMO conjugating enzyme, and reveal that SUMOylation machinery is enriched in neural crest precursors and plays a critical role in NC development. We demonstrate that Pax7 becomes SUMOylated and identify an essential role for lysine 85 (K85) in Pax7-SUMOylation. Despite high conservation surrounding K85 amongst Pax genes, we were unable to identify SUMOylation of other Pax proteins tested, including Pax3. Using a non-SUMOylatable Pax7 variant (K85 X R), we demonstrate that SUMOylation is essential for the function of Pax7 in neural crest development, C2C12 myogenic differentiation, and transcriptional transactivation. Our study provides new mechanistic insight into the molecular regulation of Pax7's function by SUMOylation in neural crest and muscle development.
Authors:
Zhidong Luan; Ying Liu; Timothy J Stuhlmiller; Jonathan Marquez; Martín I García-Castro
Related Documents :
23177988 - Sodium-sulfate/carboxylate cotransporters (slc13).
24704238 - Overfeeding energy upregulates peroxisome proliferator-activated receptor (ppar)γ-cont...
24548348 - Analysis of the floral transcriptome of tarenaya hassleriana (cleomaceae), a member of ...
24398598 - Comprehensive expression analysis of rice armadillo gene family during abiotic stress a...
4127628 - Temperature-sensitive nonsense mutations in essential genes of escherichia coli.
8590738 - Cell lines for the production of recombinant adeno-associated virus.
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-12-18
Journal Detail:
Title:  Cellular and molecular life sciences : CMLS     Volume:  -     ISSN:  1420-9071     ISO Abbreviation:  Cell. Mol. Life Sci.     Publication Date:  2012 Dec 
Date Detail:
Created Date:  2012-12-18     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9705402     Medline TA:  Cell Mol Life Sci     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
Department of Molecular, Cellular and Developmental Biology, Yale University, New Haven, CT, 06520-8103, USA.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Upper aerodigestive tract cancer in patients with chronic lymphocytic leukemia: incidence, stage, an...
Next Document:  Effect of Nutrient Supplementation on Atopic Dermatitis in Children: A Systematic Review of Probioti...