| SUMO modification modulates the activity of calpain-2. | |
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MedLine Citation:
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PMID: 19422794 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Small ubiquitin-like modifier (SUMO) modification has been shown to be involved in the regulation of various cellular processes including gene transcription, nucleocytoplasmic transport, cell cycle, DNA repair, stress response, and signal transduction. However, very little is known about the process of cell migration being modulated by SUMO modification. Here, we show that calpain-2, a protease involved in cell motility, can be SUMO modified at lysine residue 390. Converting the SUMO acceptor lysine residue to arginine residue significantly attenuated calpain-2 activity, correlating well with a loss of calpain-2-elicited cell motility. Accordingly, expression of SENP1 could abrogate calpain-2 sumoylation, causing an inhibition on calpain-2-dependent activity and cell motility. These results not only identify calpain-2 as a substrate for sumoylation but also provide an important role of sumoylation in regulating cell migration. |
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Authors:
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Hsueh-Chun Wang; Yen-Sung Huang; Chun-Chen Ho; Jen-Chong Jeng; Hsiu-Ming Shih |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-05-05 |
Journal Detail:
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Title: Biochemical and biophysical research communications Volume: 384 ISSN: 1090-2104 ISO Abbreviation: Biochem. Biophys. Res. Commun. Publication Date: 2009 Jul |
Date Detail:
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Created Date: 2009-05-27 Completed Date: 2009-06-19 Revised Date: 2012-07-11 |
Medline Journal Info:
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Nlm Unique ID: 0372516 Medline TA: Biochem Biophys Res Commun Country: United States |
Other Details:
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Languages: eng Pagination: 444-9 Citation Subset: IM |
Affiliation:
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Graduate Institute of Life Sciences, National Defense Medical Center, Taipei, Taiwan, ROC. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals COS Cells Calpain / genetics, metabolism* Cell Movement* Cercopithecus aethiops Endopeptidases / metabolism Humans Lysine / genetics, metabolism Mutation SUMO-1 Protein / metabolism* |
| Chemical | |
Reg. No./Substance:
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0/SUMO-1 Protein; 56-87-1/Lysine; EC 3.4.-/Endopeptidases; EC 3.4.-/SENP1 protein, human; EC 3.4.22.-/Calpain; EC 3.4.22.53/calpain 2, human |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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