| SUMO modification of the DEAD box protein p68 modulates its transcriptional activity and promotes its interaction with HDAC1. | |
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MedLine Citation:
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PMID: 17369852 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The nuclear protein p68 (also known as Ddx5) is a prototypic member of the 'DEAD box' family of RNA helicases, which has been shown to be abnormally expressed and modified in colorectal tumors and to function as an important transcriptional regulator. Here, we show that p68 is modified in vivo on a single site (K53) by the small ubiquitin-like modifier-2 (SUMO-2). We demonstrate that the SUMO E3 ligase PIAS1 interacts with p68 and enhances its SUMO modification in vivo. To determine the functional consequences of SUMO modification, we compared the transcriptional activity of p68 and a K53R mutant that could not be SUMO-modified. Our data show that SUMO modification enhances p68 transcriptional repression activity and inhibits the ability of p68 to function as a coactivator of p53. These findings may be explained by the ability of wild type, but not K53R p68, to alter the modification state of chromatin by recruitment of histone deacetylase 1 (HDAC1). |
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Authors:
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A-M F Jacobs; S M Nicol; R G Hislop; E G Jaffray; R T Hay; F V Fuller-Pace |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2007-03-19 |
Journal Detail:
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Title: Oncogene Volume: 26 ISSN: 0950-9232 ISO Abbreviation: Oncogene Publication Date: 2007 Aug |
Date Detail:
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Created Date: 2007-08-30 Completed Date: 2007-10-11 Revised Date: 2009-11-19 |
Medline Journal Info:
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Nlm Unique ID: 8711562 Medline TA: Oncogene Country: England |
Other Details:
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Languages: eng Pagination: 5866-76 Citation Subset: IM |
Affiliation:
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Cancer Biology Group, Division of Pathology and Neuroscience, University of Dundee, Ninewells Hospital and Medical School, Ninewells Drive, Dundee, Tayside, UK. |
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals COS Cells Cell Line, Tumor Cell Nucleus / metabolism Cercopithecus aethiops DEAD-box RNA Helicases / metabolism* Gene Expression Regulation, Neoplastic* Hela Cells Histone Deacetylase 1 Histone Deacetylases / metabolism* Humans Protein Binding Protein Inhibitors of Activated STAT / metabolism Small Ubiquitin-Related Modifier Proteins / metabolism* Transcription, Genetic Tumor Suppressor Protein p53 / metabolism |
| Chemical | |
Reg. No./Substance:
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0/PIAS1 protein, human; 0/Protein Inhibitors of Activated STAT; 0/Small Ubiquitin-Related Modifier Proteins; 0/TP53 protein, human; 0/Tumor Suppressor Protein p53; EC 3.5.1.98/HDAC1 protein, human; EC 3.5.1.98/Histone Deacetylase 1; EC 3.5.1.98/Histone Deacetylases; EC 3.6.1.-/DEAD-box RNA Helicases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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