Document Detail


STR and HLA Mutations or Losses Confound Engraftment and Typing Analysis in Hematopoietic Stem Cell Transplants.
MedLine Citation:
PMID:  21463659     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Clonal chromosomal abnormalities are often found in the tumor cells of patients with malignancies. This can cause down-regulation of human leukocyte antigen (HLA) and instability of short tandem repeat (STR) DNA sequences, confounding HLA typing and/or engraftment analysis in hematopoietic stem cell transplants (HSCT). We describe here the abnormalities observed during testing of 600 HSCT patients. HLA molecular typing was performed by reference strand conformational analyses (RSCA) and/or sequence based typing (SBT). STR testing was performed with 10-16 STR primer sets, following 1- 4 informative loci in each patient. Eight patients showed either loss of heterozygosity (LOH) (4 STR, 3 HLA) or STR length mutation (n=1), and 5 of the 8 showed correlative cytogenetic abnormalities. Diagnoses were acute myelogenous leukemia (AML) (n=7) or myelofibrosis (n =1), yielding an 11% incidence of these chromosomal abnormalities among the subset of 72 AML/ Myelofibrosis HSCT patients. These results highlight some of the problems encountered and the possibility for interpretive errors that can arise when analyzing molecular typing and engraftment data, particularly among AML/ Myelofibrosis patients.
Authors:
Shalini Pereira; Tamara Vayntrub; Debra D Hiraki; Athena M Cherry; Sally Arai; Christopher C Dvorak; F Carl Grumet
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-4-1
Journal Detail:
Title:  Human immunology     Volume:  -     ISSN:  1879-1166     ISO Abbreviation:  -     Publication Date:  2011 Apr 
Date Detail:
Created Date:  2011-4-5     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8010936     Medline TA:  Hum Immunol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2011. Published by Elsevier Inc.
Affiliation:
Clinical Research Division, Fred Hutchinson Cancer Research Center and the Department of Laboratory Medicine, University of Washington, Seattle.
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