Document Detail


STM studies of LB films of amphiphilic polyelectrolytes with antibodies and enzymes.
MedLine Citation:
PMID:  10024974     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The structure of LB films of protein-polyelectrolyte complexes transferred onto the pyrographite surface was studied by STM. The images of the films obtained at various protein concentrations in the water subphase and different values of surface pressure were captured. The topology of the surface covered by one or three layers of the films studied was investigated. It is shown that at a protein concentration of 1 mg/ml in the water subphase, the films are composed of aggregated protein molecules, and their structure has an insular character. An increase in the number of transferred layers up to three results in a virtually complete covering of the surface. A decrease in the protein concentration in the water subphase to 1 microgram/ml enabled us to prepare films consisting of individual non-aggregated protein molecules.
Authors:
A B Pavelev; I N Kurochkin; S F Chernov
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Membrane & cell biology     Volume:  12     ISSN:  1023-6597     ISO Abbreviation:  Membr Cell Biol     Publication Date:  1998  
Date Detail:
Created Date:  1999-05-19     Completed Date:  1999-05-19     Revised Date:  2000-12-18    
Medline Journal Info:
Nlm Unique ID:  9517472     Medline TA:  Membr Cell Biol     Country:  SWITZERLAND    
Other Details:
Languages:  eng     Pagination:  427-34     Citation Subset:  IM    
Affiliation:
Research Center of Molecular Diagnostics and Therapy, Moscow, Russia.
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MeSH Terms
Descriptor/Qualifier:
Antibodies / analysis*
Electrolytes / chemistry
Enzymes / analysis*
Glucose Oxidase / analysis
Membrane Proteins / chemistry,  ultrastructure*
Microscopy, Scanning Tunneling*
Monoamine Oxidase / analysis
Chemical
Reg. No./Substance:
0/Antibodies; 0/Electrolytes; 0/Enzymes; 0/Membrane Proteins; EC 1.1.3.4/Glucose Oxidase; EC 1.4.3.4/Monoamine Oxidase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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