Document Detail


STEM mode in the SEM: a practical tool for nanotoxicology.
MedLine Citation:
PMID:  21090920     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The addition of a transmitted electron detector to a scanning electron microscope (SEM) allows the recording of bright and dark field scanning transmission electron microscope (STEM) images and the corresponding in-lens secondary electron images from the same region of a thin sample. These combined imaging techniques have been applied here to the analysis of ultrathin sections of cells exposed in vitro to nanomaterials for toxicology investigation. Electron microscopy in general permits the exact nature of the interaction of nanomaterials and cells to be elucidated, and in addition the use of STEM mode in the SEM enables the easy identification and exclusion of artefacts produced by ultramicrotome sectioning. The imaging and analysis obtained by using the STEM mode in the SEM configuration from three different nanomaterial systems of importance (iron oxide nanoparticles, single-walled carbon nanotubes and cadmium selenide quantum dots) indicate that it is a simple, practical and cost-effective tool for nanotoxicological research.
Authors:
Nicole Hondow; John Harrington; Rik Brydson; Shareen H Doak; Neenu Singh; Bella Manshian; Andy Brown
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-11-19
Journal Detail:
Title:  Nanotoxicology     Volume:  5     ISSN:  1743-5404     ISO Abbreviation:  Nanotoxicology     Publication Date:  2011 Jun 
Date Detail:
Created Date:  2011-05-25     Completed Date:  2011-09-13     Revised Date:  2014-02-20    
Medline Journal Info:
Nlm Unique ID:  101233132     Medline TA:  Nanotoxicology     Country:  England    
Other Details:
Languages:  eng     Pagination:  215-27     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Line
Humans
Microscopy, Electron, Scanning / instrumentation,  methods
Microscopy, Electron, Scanning Transmission / instrumentation*,  methods*
Nanostructures / chemistry,  toxicity*,  ultrastructure
Toxicology / instrumentation*,  methods*
Grant Support
ID/Acronym/Agency:
G0700865//Medical Research Council; G0700865/82417//Medical Research Council

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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