Document Detail

STAT5a-targeting miRNA enhances chemosensitivity to cisplatin and 5-fluorouracil in human colorectal cancer cells.
MedLine Citation:
PMID:  22367509     Owner:  NLM     Status:  MEDLINE    
Signal transducers and activators of transcription 5 (STAT5) has been shown to be involved in a variety of cellular processes, including survival, proliferation, invasion, angiogenesis and immune evasion and is frequently overexpressed in human solid tumors and blood malignancies. The aim of this study was to investigate the role of STAT5a in colorectal cancer (CRC) progression. We inhibited the expression of STAT5a using lentivirus-mediated artificial microRNA (miRNA) interference in vitro and investigated the viability of CRC cells by CCK-8 assay. We observed the cell viability after treatment with cisplatin (CDDP) or 5-fluorouracil (5-Fu) by CCK-8 assay, and the apoptosis induced by chemotherapy using flow cytometric analysis and Annexin V RFP staining assay. We inhibited the mRNA expression by 54% and the protein expression by 60% of STAT5 by RNA interference targeting STAT5a. Cell viability assays showed that inhibition of STAT5a did not affect the viability of SW1116 cells. However, we found that inhibition of STAT5a restored the sensitivity of SW1116 cells to CDDP and 5-Fu. In additional experiments, we found that inhibition of STAT5a significantly promoted CRC cell apoptosis by CDDP and 5-Fu. In the present study, we found that inhibition of STAT5a promotes apoptosis of CRC cells induced by chemotherapy drugs, such as CDDP or 5-Fu. These results suggest that inhibition of STAT5a may serve as a potential new target for CRC treatment.
Xuan Hong; Gongyan Chen; Meng Wang; Changjie Lou; Yinling Mao; Zhiwei Li; Yanqiao Zhang
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Publication Detail:
Type:  Journal Article     Date:  2012-02-21
Journal Detail:
Title:  Molecular medicine reports     Volume:  5     ISSN:  1791-3004     ISO Abbreviation:  Mol Med Rep     Publication Date:  2012 May 
Date Detail:
Created Date:  2012-03-12     Completed Date:  2012-07-03     Revised Date:  2013-02-22    
Medline Journal Info:
Nlm Unique ID:  101475259     Medline TA:  Mol Med Rep     Country:  Greece    
Other Details:
Languages:  eng     Pagination:  1215-9     Citation Subset:  IM    
Department of Respiratory Medical Oncology, The Third Affiliated Hospital of Harbin Medical University, Harbin, PR China.
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MeSH Terms
Annexin A5 / biosynthesis,  genetics
Antimetabolites, Antineoplastic / pharmacology*
Apoptosis / drug effects,  genetics
Cell Line, Tumor
Cell Survival / drug effects,  genetics
Cisplatin / pharmacology*
Colorectal Neoplasms / drug therapy*,  genetics,  metabolism,  pathology
Drug Resistance, Neoplasm / drug effects*,  genetics
Fluorouracil / pharmacology*
MicroRNAs / biosynthesis*,  genetics
RNA Interference
RNA, Neoplasm / biosynthesis*,  genetics
STAT5 Transcription Factor / biosynthesis*,  genetics
Transduction, Genetic
Tumor Suppressor Proteins / biosynthesis*,  genetics
Reg. No./Substance:
0/Annexin A5; 0/Antimetabolites, Antineoplastic; 0/MicroRNAs; 0/RNA, Neoplasm; 0/STAT5 Transcription Factor; 0/STAT5A protein, human; 0/Tumor Suppressor Proteins; 15663-27-1/Cisplatin; 51-21-8/Fluorouracil

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