Document Detail

STAT3 blockade with shRNA enhances radiosensitivity in Hep-2 human laryngeal squamous carcinoma cells.
MedLine Citation:
PMID:  20043094     Owner:  NLM     Status:  MEDLINE    
Signal transducer and activator of transcription 3 (STAT3) is an oncogene aberrantly activated in many human tumors. We studied whether radiation combined with STAT3 siRNA enhances radiosensitivity of Hep-2 human laryngeal squamous carcinoma cells (Hep-2 cells). Firstly, STAT3 targeting recombinant plasmid was constructed. Hep-2 cells were transfected with expression vector of STAT3 siRNA using Lipofectamine 2000. Semiquantitive RT-PCR detected effective STAT3 mRNA down-regulation by STAT3 siRNA. Secondly, Hep-2 cells were radiated with different doses of gamma-rays after transfection with STAT3 small interference RNA (siRNA). MTT assay showed cell proliferation decreased significantly (P<0.05) after STAT3 siRNA transfection combined with radiation. Thirdly, flow cytometry (FCM) demonstrated that cell apoptosis of combined treatment group increased significantly (P<0.05) and exhibited time dependency after 6 Gy irradiation (P<0.05). Simultaneously, STAT3, p-STAT3, Bcl-2, VEGF, p53 protein levels decreased in Hep-2 cells, with positive correlations between level of p-STAT3 and levels of Bcl-2, VEGF, p53, respectively (r=0.974, 0.988, 0.976, all P<0.01). Above all, specific siRNA targeting STAT3 gene is able to enhance the radiosensitivity in Hep-2 cells by regulating expression of Bcl-2, VEGF and p53 proteins.
Xiaoming Li; Hairu Wang; Xiuying Lu; Bin Di
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Publication Detail:
Type:  Evaluation Studies; Journal Article    
Journal Detail:
Title:  Oncology reports     Volume:  23     ISSN:  1791-2431     ISO Abbreviation:  Oncol. Rep.     Publication Date:  2010 Feb 
Date Detail:
Created Date:  2009-12-31     Completed Date:  2010-03-30     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9422756     Medline TA:  Oncol Rep     Country:  Greece    
Other Details:
Languages:  eng     Pagination:  345-53     Citation Subset:  IM    
Department of Otolaryngology-Head and Neck Surgery, Bethune International Peace Hospital, Shijiazhuang, Hebei Province, PR China.
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MeSH Terms
Apoptosis / drug effects,  radiation effects
Base Sequence
Carcinoma, Squamous Cell / genetics,  pathology,  radiotherapy*
Cell Line, Tumor
Cell Survival / drug effects,  radiation effects
Gene Expression Regulation, Neoplastic / drug effects
Laryngeal Neoplasms / genetics,  pathology,  radiotherapy*
Molecular Sequence Data
Proto-Oncogene Proteins c-bcl-2 / genetics,  physiology
RNA, Small Interfering / genetics,  pharmacology*
Radiation Dosage
Radiation Tolerance / drug effects*,  genetics
Radiation-Sensitizing Agents / pharmacology
STAT3 Transcription Factor / antagonists & inhibitors*,  genetics
Tumor Suppressor Protein p53 / genetics,  physiology
Vascular Endothelial Growth Factor A / genetics,  physiology
Reg. No./Substance:
0/Proto-Oncogene Proteins c-bcl-2; 0/RNA, Small Interfering; 0/Radiation-Sensitizing Agents; 0/STAT3 Transcription Factor; 0/STAT3 protein, human; 0/Tumor Suppressor Protein p53; 0/VEGFA protein, human; 0/Vascular Endothelial Growth Factor A

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