| STAT3 blockade with shRNA enhances radiosensitivity in Hep-2 human laryngeal squamous carcinoma cells. | |
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MedLine Citation:
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PMID: 20043094 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Signal transducer and activator of transcription 3 (STAT3) is an oncogene aberrantly activated in many human tumors. We studied whether radiation combined with STAT3 siRNA enhances radiosensitivity of Hep-2 human laryngeal squamous carcinoma cells (Hep-2 cells). Firstly, STAT3 targeting recombinant plasmid was constructed. Hep-2 cells were transfected with expression vector of STAT3 siRNA using Lipofectamine 2000. Semiquantitive RT-PCR detected effective STAT3 mRNA down-regulation by STAT3 siRNA. Secondly, Hep-2 cells were radiated with different doses of gamma-rays after transfection with STAT3 small interference RNA (siRNA). MTT assay showed cell proliferation decreased significantly (P<0.05) after STAT3 siRNA transfection combined with radiation. Thirdly, flow cytometry (FCM) demonstrated that cell apoptosis of combined treatment group increased significantly (P<0.05) and exhibited time dependency after 6 Gy irradiation (P<0.05). Simultaneously, STAT3, p-STAT3, Bcl-2, VEGF, p53 protein levels decreased in Hep-2 cells, with positive correlations between level of p-STAT3 and levels of Bcl-2, VEGF, p53, respectively (r=0.974, 0.988, 0.976, all P<0.01). Above all, specific siRNA targeting STAT3 gene is able to enhance the radiosensitivity in Hep-2 cells by regulating expression of Bcl-2, VEGF and p53 proteins. |
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Authors:
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Xiaoming Li; Hairu Wang; Xiuying Lu; Bin Di |
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Publication Detail:
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Type: Evaluation Studies; Journal Article |
Journal Detail:
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Title: Oncology reports Volume: 23 ISSN: 1791-2431 ISO Abbreviation: Oncol. Rep. Publication Date: 2010 Feb |
Date Detail:
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Created Date: 2009-12-31 Completed Date: 2010-03-30 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9422756 Medline TA: Oncol Rep Country: Greece |
Other Details:
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Languages: eng Pagination: 345-53 Citation Subset: IM |
Affiliation:
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Department of Otolaryngology-Head and Neck Surgery, Bethune International Peace Hospital, Shijiazhuang, Hebei Province, PR China. xmlmo@126.com |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Apoptosis
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drug effects,
radiation effects Base Sequence Carcinoma, Squamous Cell / genetics, pathology, radiotherapy* Cell Line, Tumor Cell Survival / drug effects, radiation effects Gene Expression Regulation, Neoplastic / drug effects Humans Laryngeal Neoplasms / genetics, pathology, radiotherapy* Molecular Sequence Data Proto-Oncogene Proteins c-bcl-2 / genetics, physiology RNA, Small Interfering / genetics, pharmacology* Radiation Dosage Radiation Tolerance / drug effects*, genetics Radiation-Sensitizing Agents / pharmacology STAT3 Transcription Factor / antagonists & inhibitors*, genetics Transfection Tumor Suppressor Protein p53 / genetics, physiology Vascular Endothelial Growth Factor A / genetics, physiology |
| Chemical | |
Reg. No./Substance:
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0/Proto-Oncogene Proteins c-bcl-2; 0/RNA, Small Interfering; 0/Radiation-Sensitizing Agents; 0/STAT3 Transcription Factor; 0/STAT3 protein, human; 0/Tumor Suppressor Protein p53; 0/VEGFA protein, human; 0/Vascular Endothelial Growth Factor A |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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