Document Detail

SSU1 mediates sulphite efflux in Saccharomyces cerevisiae.
MedLine Citation:
PMID:  10870099     Owner:  NLM     Status:  MEDLINE    
Ssu1p, a plasma membrane protein involved in sulphite metabolism in Saccharomyces cerevisiae, was found to be required for efficient sulphite efflux. An SSU1 null mutant accumulated significantly more sulphite than wild-type, whereas cells expressing multicopy SSU1 accumulated significantly less. Cells expressing FZF1-4, a dominant allele of a transcriptional activator of SSU1 that confers sulphite resistance, also accumulated less sulphite. beta-galactosidase activity in the FZF1-4 strain carrying an SSU1::lacZ fusion was found to be 8.5-fold higher than in a strain carrying wild-type FZF1, confirming that the heightened resistance was correlated with hyperactivation of SSU1. Multicopy SSU1 was also found to increase the sulphite resistance of a number of unrelated sulphite-sensitive strains by a factor of 3- to 8-fold. Rates of efflux of free sulphite from cells expressing multicopy SSU1 or FZF1-4 were significantly greater than that from wild-type or from a SSU1 null mutant. Rates of efflux of bound sulphite from wild-type, a SSU1 null mutant, a FZF1-4 mutant, or cells expressing multicopy SSU1 were not significantly different, suggesting that Ssu1p specifically mediates efflux of the free form of sulphite.
H Park; A T Bakalinsky
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Yeast (Chichester, England)     Volume:  16     ISSN:  0749-503X     ISO Abbreviation:  Yeast     Publication Date:  2000 Jul 
Date Detail:
Created Date:  2000-10-05     Completed Date:  2000-10-05     Revised Date:  2005-11-17    
Medline Journal Info:
Nlm Unique ID:  8607637     Medline TA:  Yeast     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  881-8     Citation Subset:  IM    
Copyright Information:
Copyright 2000 John Wiley & Sons, Ltd.
Department of Food Science and Technology, Oregon State University, OR 97331-6602, Corvallis, USA.
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MeSH Terms
Anion Transport Proteins*
Biological Transport
Drug Resistance, Microbial
Fungal Proteins / genetics,  metabolism*
Gene Expression Regulation, Fungal
Membrane Proteins / genetics,  metabolism*
Oxidoreductases Acting on Sulfur Group Donors / metabolism
Saccharomyces cerevisiae / drug effects,  genetics,  growth & development,  metabolism*
Saccharomyces cerevisiae Proteins*
Sulfites / metabolism*,  pharmacology
Trans-Activators / genetics,  metabolism
Transcription Factors*
beta-Galactosidase / metabolism
Reg. No./Substance:
0/Anion Transport Proteins; 0/FZF1 protein, S cerevisiae; 0/Fungal Proteins; 0/Membrane Proteins; 0/Saccharomyces cerevisiae Proteins; 0/Ssu1 protein, S cerevisiae; 0/Sulfites; 0/Trans-Activators; 0/Transcription Factors; EC 1.8.-/Oxidoreductases Acting on Sulfur Group Donors; EC

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