| SSU1 mediates sulphite efflux in Saccharomyces cerevisiae. | |
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MedLine Citation:
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PMID: 10870099 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Ssu1p, a plasma membrane protein involved in sulphite metabolism in Saccharomyces cerevisiae, was found to be required for efficient sulphite efflux. An SSU1 null mutant accumulated significantly more sulphite than wild-type, whereas cells expressing multicopy SSU1 accumulated significantly less. Cells expressing FZF1-4, a dominant allele of a transcriptional activator of SSU1 that confers sulphite resistance, also accumulated less sulphite. beta-galactosidase activity in the FZF1-4 strain carrying an SSU1::lacZ fusion was found to be 8.5-fold higher than in a strain carrying wild-type FZF1, confirming that the heightened resistance was correlated with hyperactivation of SSU1. Multicopy SSU1 was also found to increase the sulphite resistance of a number of unrelated sulphite-sensitive strains by a factor of 3- to 8-fold. Rates of efflux of free sulphite from cells expressing multicopy SSU1 or FZF1-4 were significantly greater than that from wild-type or from a SSU1 null mutant. Rates of efflux of bound sulphite from wild-type, a SSU1 null mutant, a FZF1-4 mutant, or cells expressing multicopy SSU1 were not significantly different, suggesting that Ssu1p specifically mediates efflux of the free form of sulphite. |
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Authors:
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H Park; A T Bakalinsky |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Yeast (Chichester, England) Volume: 16 ISSN: 0749-503X ISO Abbreviation: Yeast Publication Date: 2000 Jul |
Date Detail:
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Created Date: 2000-10-05 Completed Date: 2000-10-05 Revised Date: 2005-11-17 |
Medline Journal Info:
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Nlm Unique ID: 8607637 Medline TA: Yeast Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 881-8 Citation Subset: IM |
Copyright Information:
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Copyright 2000 John Wiley & Sons, Ltd. |
Affiliation:
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Department of Food Science and Technology, Oregon State University, OR 97331-6602, Corvallis, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Anion Transport Proteins* Biological Transport Drug Resistance, Microbial Fungal Proteins / genetics, metabolism* Gene Expression Regulation, Fungal Membrane Proteins / genetics, metabolism* Mutation Oxidoreductases Acting on Sulfur Group Donors / metabolism Saccharomyces cerevisiae / drug effects, genetics, growth & development, metabolism* Saccharomyces cerevisiae Proteins* Sulfites / metabolism*, pharmacology Trans-Activators / genetics, metabolism Transcription Factors* beta-Galactosidase / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Anion Transport Proteins; 0/FZF1 protein, S cerevisiae; 0/Fungal Proteins; 0/Membrane Proteins; 0/Saccharomyces cerevisiae Proteins; 0/Ssu1 protein, S cerevisiae; 0/Sulfites; 0/Trans-Activators; 0/Transcription Factors; EC 1.8.-/Oxidoreductases Acting on Sulfur Group Donors; EC 3.2.1.23/beta-Galactosidase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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