Document Detail

SRY sequence in maternal plasma: Implications for non-invasive prenatal diagnosis: First report from India.
MedLine Citation:
PMID:  22754228     Owner:  NLM     Status:  PubMed-not-MEDLINE    
AIM: The presence of circulatory cell-free fetal DNA in maternal plasma has found new applications in non-invasive risk-free prenatal diagnosis.
MATERIALS AND METHODS: We made use of a size separation approach along with real time polymerase chain reaction (PCR) to evaluate the use of fetal DNA in the detection of the sex of the fetus. Cell-free fetal DNA was isolated from the plasma of 30 women (10-20 weeks gestation) using a size separation approach. We made use of Taq Man Chemistry and real time PCR using primers and probes for GAPDH and SRY.
RESULTS: Only 24 cases could be studied as there was no amplification in six cases. Fetal sex was accurately determined in all of the 24 cases wherein 19 women were carrying male fetuses and five women were carrying female fetuses. An increase in the amount of fetal DNA was observed with an increase in the gestational age.
CONCLUSIONS: Real time PCR analysis is a highly sensitive and accurate tool for non-invasive prenatal diagnosis, allowing detection of the sex of the fetus as early as 10 weeks of gestation. Non-invasive prenatal diagnosis eliminates the risk of fetal loss associated with the invasive procedure.
Edna D'Souza; Sona Nair; Anita Nadkarni; Kanjaksha Ghosh; Roshan B Colah
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Indian journal of human genetics     Volume:  18     ISSN:  0971-6866     ISO Abbreviation:  Indian J Hum Genet     Publication Date:  2012 Jan 
Date Detail:
Created Date:  2012-07-03     Completed Date:  2012-10-02     Revised Date:  2013-05-30    
Medline Journal Info:
Nlm Unique ID:  101223637     Medline TA:  Indian J Hum Genet     Country:  India    
Other Details:
Languages:  eng     Pagination:  87-90     Citation Subset:  -    
National Institute of Immunohaematology (ICMR), New Multistoried Building, K.E.M. Hospital Campus, Parel, Mumbai, India.
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