| SREA is involved in regulation of siderophore biosynthesis, utilization and uptake in Aspergillus nidulans. | |
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MedLine Citation:
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PMID: 11555288 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Under conditions of low iron availability, most fungi excrete siderophores in order to mobilize extracellular iron. We show that lack of the GATA-type transcription factor SREA in Aspergillus nidulans not only leads to derepression of siderophore biosynthesis but also to deregulation of siderophore-bound iron uptake and ornithine esterase expression. Furthermore, SREA deficiency causes increased accumulation of ferricrocin, the siderophore responsible for intracellular iron storage. In sreA deletion strains, extracellular siderophore production is derepressed but still regulated negatively by iron availability, indicating the presence of an additional iron-regulatory mechanism. In contrast, iron affects ferricrocin accumulation in a positive way, suggesting a protective role for this siderophore in detoxification of intracellular iron excess. The harmfulness of deregulated iron uptake in this mutant is demonstrated by increased expression of genes encoding the antioxidative enzymes catalase CATB and the superoxide dismutases SODA and SODB. It is noteworthy that iron starvation was found to repress catB expression in wild-type (wt) and SREA-deficient strains, consistent with catB being subject to SREA-independent iron regulation. Differential display led to the identification of putative SREA target genes amcA and mirA. The deduced MIRA amino acid sequence displays significant similarity to recently characterized siderophore permeases of Saccharomyces cerevisiae. amcA encodes a putative mitochondrial carrier for the siderophore precursor ornithine, indicating cross-regulation of siderophore and ornithine metabolism. |
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Authors:
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H Oberegger; M Schoeser; I Zadra; B Abt; H Haas |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Molecular microbiology Volume: 41 ISSN: 0950-382X ISO Abbreviation: Mol. Microbiol. Publication Date: 2001 Sep |
Date Detail:
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Created Date: 2001-09-13 Completed Date: 2002-02-11 Revised Date: 2009-12-11 |
Medline Journal Info:
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Nlm Unique ID: 8712028 Medline TA: Mol Microbiol Country: England |
Other Details:
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Languages: eng Pagination: 1077-89 Citation Subset: IM |
Affiliation:
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Department of Microbiology, Medical School, University of Innsbruck, Fritz-Pregl-Str. 3, A-6020 Innsbruck, Austria. |
| Data Bank Information | |
Bank Name/Acc. No.:
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GENBANK/AY027563; AY027565 |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Aspergillus nidulans / genetics, growth & development, metabolism* Bacterial Proteins / chemistry, genetics*, metabolism Carrier Proteins / chemistry, genetics*, metabolism Cloning, Molecular Esterases / metabolism Ferrichrome / analogs & derivatives*, metabolism Fungal Proteins / chemistry, genetics*, metabolism GATA Transcription Factors Gene Expression Regulation, Fungal* Iron / metabolism Membrane Transport Proteins / chemistry, genetics*, metabolism Mitochondrial Membrane Transport Proteins Mitochondrial Proteins / chemistry, genetics*, metabolism Molecular Sequence Data Ornithine / metabolism Repressor Proteins / genetics, metabolism* Sequence Alignment Sequence Analysis, DNA Siderophores / biosynthesis*, metabolism* Superoxide Dismutase / genetics, metabolism |
| Chemical | |
Reg. No./Substance:
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0/Bacterial Proteins; 0/Carrier Proteins; 0/Fungal Proteins; 0/GATA Transcription Factors; 0/MIRA protein, Emericella nidulans; 0/Membrane Transport Proteins; 0/Mitochondrial Membrane Transport Proteins; 0/Mitochondrial Proteins; 0/Repressor Proteins; 0/SREA protein, Aspergillus nidulans; 0/Siderophores; 0/SodB protein, Bacteria; 15630-64-5/Ferrichrome; 23086-46-6/ferricrocin; 7006-33-9/Ornithine; 7439-89-6/Iron; EC 1.15.1.1/Superoxide Dismutase; EC 3.1.-/Esterases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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