Document Detail

SR-BI, CD36, and caveolin-1 contribute positively to cholesterol efflux in hepatic cells.
MedLine Citation:
PMID:  20629037     Owner:  NLM     Status:  MEDLINE    
In non-hepatic cells, scavenger receptor class B type I (SR-BI), cluster of differentiation 36 (CD36), and caveolin-1 were described as mediators of cholesterol efflux, the first step of reverse cholesterol transport (RCT). Stable transformants of HepG2 cells overexpressing SR-BI, CD36, or caveolin-1 were generated, as well as cells overexpressing both caveolin-1 and SR-BI or caveolin-1 and CD36 in order to address the effect of caveolin-1 on both receptor activities. These cells were analyzed for their ability to efflux cholesterol to HDL(3). Our results show that overexpressing SR-BI, CD36, or caveolin-1 increases cholesterol efflux by 106, 92, and 48%, respectively. Moreover, the dual overexpressions of caveolin-1 and SR-BI or caveolin-1 and CD36 lead to a more prominent increase in cholesterol efflux. Studies were also conducted with primary cultures of SR-BI knockout (KO), CD36 KO, and SR-BI/CD36 double-KO (dKO) mice. SR-BI KO and SR-BI/CD36 dKO hepatic cells show 41 and 56% less cholesterol efflux, respectively, than normal hepatic cells. No significant difference was observed between the efflux of normal and CD36 KO cells. The difference between the role of human and murine CD36 correlated with the absence of CD36 dimers in mouse caveolae/rafts. Overall, our results show that SR-BI is clearly involved in cholesterol efflux in mouse and human hepatic cells, while CD36 plays a significant role in human cells.
To Quyen Truong; Dominique Aubin; Louise Falstrault; Mathieu R Brodeur; Louise Brissette
Related Documents :
10634807 - The heparin-binding proteins apolipoprotein e and lipoprotein lipase enhance cellular p...
12659867 - Binding and internalization of helicobacter pylori vaca via cellular lipid rafts in epi...
10948207 - Perilipin a increases triacylglycerol storage by decreasing the rate of triacylglycerol...
20844007 - Deficiency of scavenger receptor class b type i negatively affects progesterone secreti...
6576817 - Iron storage in ferritin following intracellular hemoglobin denaturation in erythroleuk...
23641717 - Diffuse idiopathic pulmonary neuroendocrine cell hyperplasia: case report and review of...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cell biochemistry and function     Volume:  28     ISSN:  1099-0844     ISO Abbreviation:  Cell Biochem. Funct.     Publication Date:  2010 Aug 
Date Detail:
Created Date:  2010-08-30     Completed Date:  2010-12-13     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8305874     Medline TA:  Cell Biochem Funct     Country:  England    
Other Details:
Languages:  eng     Pagination:  480-9     Citation Subset:  IM    
Département des Sciences Biologiques, Université du Québec à Montréal, Montréal, Québec, Canada.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Antigens, CD36 / genetics,  metabolism*
Caveolin 1 / genetics,  metabolism*
Cells, Cultured
Cholesterol / metabolism*
Hep G2 Cells
Hepatocytes / metabolism*
Mice, Knockout
Scavenger Receptors, Class B / genetics,  metabolism*
Grant Support
//Canadian Institutes of Health Research
Reg. No./Substance:
0/Antigens, CD36; 0/Caveolin 1; 0/SCARB1 protein, human; 0/Scarb1 protein, mouse; 0/Scavenger Receptors, Class B; 57-88-5/Cholesterol

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Selection and optimization of MCF-7 cell line for screening selective inhibitors of 11beta-hydroxyst...
Next Document:  Chemiluminescence sensor for sulfonylurea herbicide using molecular imprinted microspheres as recogn...