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SPI-1-encoded type III secretion system of Salmonella enterica is required for the suppression of porcine alveolar macrophage cytokine expression.
MedLine Citation:
PMID:  21314975     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
ABSTRACT: Genes localized at Salmonella pathogenicity island-1 (SPI-1) are involved in Salmonella enterica invasion of host non-professional phagocytes. Interestingly, in macrophages, SPI-1-encoded proteins, in addition to invasion, induce cell death via activation of caspase-1 which also cleaves proIL-1β and proIL-18, precursors of 2 proinflammatory cytokines. In this study we were therefore interested in whether SPI-1-encoded type III secretion system (T3SS) may influence proinflammatory response of macrophages. To test this hypothesis, we infected primary porcine alveolar macrophages with wild-type S. Typhimurium and S. Enteritidis and their isogenic SPI-1 deletion mutants. ΔSPI1 mutants of both serovars invaded approx. 5 times less efficiently than the wild-type strains and despite this, macrophages responded to the infection with ΔSPI1 mutants by increased expression of proinflammatory cytokines IL-1β, IL-8, TNFα, IL-23α and GM-CSF. Identical macrophage responses to that induced by the ΔSPI1 mutants were also observed to the infection with sipB but not the sipA mutant. The hilA mutant exhibited an intermediate phenotype between the ΔSPI1 mutant and the wild-type S. Enteritidis. Our results showed that the SPI-1-encoded T3SS is required not only for cell invasion but in macrophages also for the suppression of early proinflammatory cytokine expression.
Authors:
Barbora Pavlova; Jiri Volf; Petra Ondrackova; Jan Matiasovic; Hana Stepanova; Magdalena Crhanova; Daniela Karasova; Martin Faldyna; Ivan Rychlik
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Publication Detail:
Type:  Journal Article     Date:  2011-01-24
Journal Detail:
Title:  Veterinary research     Volume:  42     ISSN:  0928-4249     ISO Abbreviation:  Vet. Res.     Publication Date:    2011 Jan-Feb
Date Detail:
Created Date:  2011-02-14     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9309551     Medline TA:  Vet Res     Country:  England    
Other Details:
Languages:  eng     Pagination:  16     Citation Subset:  IM    
Affiliation:
Veterinary Research Institute, Brno, Czech Republic. rychlik@vri.cz.
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