Document Detail

SPEC disc solid-phase extraction for rapid broad-spectrum drug screening in urine.
MedLine Citation:
PMID:  10732947     Owner:  NLM     Status:  MEDLINE    
Broad-spectrum drug screening requires that all relevant substances be isolated, detected, and identified, regardless of their structure and/or polarity. To this end, systematic solid-phase extraction (SPE) approaches for drug isolation from biological fluids are required. Because speed and cost effectiveness are key issues in analytical toxicology, we have evaluated a disc-format extraction device for this purpose and compared the latter with an existing packed-bed column-format method. The discs were SPEC.PLUS.C18AR/MP3 cartridges with 10-mL solvent reservoirs, providing hydrophobic and cation exchange interactions. Blank human urine was spiked at 2 microg/mL with a selection of acidic, neutral, and basic drugs representing a variety of relevant drug classes. Urine specimens (2 mL) were diluted with 2 mL 0.1 M phosphate buffer (pH 5.0) and then applied to the preconditioned disc. Washing was done with 1 mL water. Acidic and neutral drugs were eluted with 1 mL ethyl acetate/acetone (1:1), and basic drugs were eluted with 1 mL ammoniated ethyl acetate. The eluates were collected separately, evaporated down to about 0.1 mL, and analyzed by gas chromatography-flame-ionization detection to check cleanliness, recoveries, and reproducibilities. The discs showed good extraction properties for all drugs and were easy to handle. Recoveries were 75-100% with coefficients of variation of around 5%. The resulting eluates showed only a few matrix interferences. As compared to our standard SPE method with packed-bed columns, the disc procedure allowed reductions in elution volumes and total processing time of approximately 60-65%.
R A de Zeeuw; J Wijsbeek; J P Franke
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Journal of analytical toxicology     Volume:  24     ISSN:  0146-4760     ISO Abbreviation:  J Anal Toxicol     Publication Date:  2000 Mar 
Date Detail:
Created Date:  2000-04-11     Completed Date:  2000-04-11     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  7705085     Medline TA:  J Anal Toxicol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  97-101     Citation Subset:  IM    
Department of Analytical Chemistry and Toxicology, University Centre for Pharmacy, Groningen, The Netherlands.
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MeSH Terms
Barbiturates / urine*
Chromatography, Gas
Chromatography, High Pressure Liquid / methods
Diazepam / urine*
Evaluation Studies as Topic
Flame Ionization
Forensic Medicine / methods
Narcotics / urine*
Phencyclidine / urine*
Reproducibility of Results
Substance Abuse Detection / methods*
Reg. No./Substance:
0/Barbiturates; 0/Narcotics; 439-14-5/Diazepam; 77-10-1/Phencyclidine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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