| SOD2-mediated effects induced by WR1065 and low-dose ionizing radiation on micronucleus formation in RKO human colon carcinoma cells. | |
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MedLine Citation:
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PMID: 21175348 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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RKO36 cells exposed to either WR1065 or 10 cGy X rays show elevated SOD2 gene expression and SOD2 enzymatic activity. Cells challenged at this time with 2 Gy exhibit enhanced radiation resistance. This phenomenon has been identified as a delayed radioprotective effect or an adaptive response when induced by thiols or low-dose radiation, respectively. In this study we investigated the relative effectiveness of both WR1065 and low-dose radiation in reducing the incidence of radiation-induced micronucleus formation in binucleated RKO36 human colon carcinoma cells. The role of SOD2 in this process was assessed by measuring changes in enzymatic activity as a function of the inducing agent used, the level of protection afforded, and the inhibitory effects of short interfering RNA (SOD2 siRNA). Both WR1065 and 10 cGy X rays effectively induced a greater than threefold elevation in SOD2 activity 24 h after exposure. Cells irradiated at this time with 2 Gy exhibited a significant resistance to micronucleus formation (P < 0.05; Student's two-tailed t test). This protective effect was significantly inhibited in cells transfected with SOD2 siRNA. SOD2 played an important role in the adaptive/delayed radioprotective response by inhibiting the initiation of a superoxide anion-induced ROS cascade leading to enhanced mitochondrial and nuclear damages. |
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Authors:
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Jeffrey S Murley; Yasushi Kataoka; Richard C Miller; Jian Jian Li; Gayle Woloschak; David J Grdina |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S. Date: 2010-11-08 |
Journal Detail:
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Title: Radiation research Volume: 175 ISSN: 1938-5404 ISO Abbreviation: Radiat. Res. Publication Date: 2011 Jan |
Date Detail:
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Created Date: 2010-12-23 Completed Date: 2011-01-20 Revised Date: 2012-09-25 |
Medline Journal Info:
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Nlm Unique ID: 0401245 Medline TA: Radiat Res Country: United States |
Other Details:
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Languages: eng Pagination: 57-65 Citation Subset: IM; S |
Affiliation:
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Department of Radiation and Cellular Oncology, The University of Chicago, Chicago, Illinois 60637, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adaptation, Physiological Cell Line, Tumor Colorectal Neoplasms / genetics* Humans Mercaptoethylamines / pharmacology* Micronuclei, Chromosome-Defective* Radiation-Protective Agents / pharmacology* Reactive Oxygen Species / metabolism Superoxide Dismutase / antagonists & inhibitors, physiology*, radiation effects Superoxides / metabolism |
| Grant Support | |
ID/Acronym/Agency:
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R01 CA132998-02/CA/NCI NIH HHS; R01-CA132998/CA/NCI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Mercaptoethylamines; 0/Radiation-Protective Agents; 0/Reactive Oxygen Species; 11062-77-4/Superoxides; 31098-42-7/WR 1065; EC 1.15.1.1/Superoxide Dismutase; EC 1.15.1.1/superoxide dismutase 2 |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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