Document Detail

SOD2-mediated effects induced by WR1065 and low-dose ionizing radiation on micronucleus formation in RKO human colon carcinoma cells.
MedLine Citation:
PMID:  21175348     Owner:  NLM     Status:  MEDLINE    
RKO36 cells exposed to either WR1065 or 10 cGy X rays show elevated SOD2 gene expression and SOD2 enzymatic activity. Cells challenged at this time with 2 Gy exhibit enhanced radiation resistance. This phenomenon has been identified as a delayed radioprotective effect or an adaptive response when induced by thiols or low-dose radiation, respectively. In this study we investigated the relative effectiveness of both WR1065 and low-dose radiation in reducing the incidence of radiation-induced micronucleus formation in binucleated RKO36 human colon carcinoma cells. The role of SOD2 in this process was assessed by measuring changes in enzymatic activity as a function of the inducing agent used, the level of protection afforded, and the inhibitory effects of short interfering RNA (SOD2 siRNA). Both WR1065 and 10 cGy X rays effectively induced a greater than threefold elevation in SOD2 activity 24 h after exposure. Cells irradiated at this time with 2 Gy exhibited a significant resistance to micronucleus formation (P < 0.05; Student's two-tailed t test). This protective effect was significantly inhibited in cells transfected with SOD2 siRNA. SOD2 played an important role in the adaptive/delayed radioprotective response by inhibiting the initiation of a superoxide anion-induced ROS cascade leading to enhanced mitochondrial and nuclear damages.
Jeffrey S Murley; Yasushi Kataoka; Richard C Miller; Jian Jian Li; Gayle Woloschak; David J Grdina
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2010-11-08
Journal Detail:
Title:  Radiation research     Volume:  175     ISSN:  1938-5404     ISO Abbreviation:  Radiat. Res.     Publication Date:  2011 Jan 
Date Detail:
Created Date:  2010-12-23     Completed Date:  2011-01-20     Revised Date:  2013-07-03    
Medline Journal Info:
Nlm Unique ID:  0401245     Medline TA:  Radiat Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  57-65     Citation Subset:  IM; S    
Department of Radiation and Cellular Oncology, The University of Chicago, Chicago, Illinois 60637, USA.
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MeSH Terms
Adaptation, Physiological
Cell Line, Tumor
Colorectal Neoplasms / genetics*
Mercaptoethylamines / pharmacology*
Micronuclei, Chromosome-Defective*
Radiation-Protective Agents / pharmacology*
Reactive Oxygen Species / metabolism
Superoxide Dismutase / antagonists & inhibitors,  physiology*,  radiation effects
Superoxides / metabolism
Grant Support
R01 CA132998-02/CA/NCI NIH HHS; R01-CA132998/CA/NCI NIH HHS
Reg. No./Substance:
0/Mercaptoethylamines; 0/Radiation-Protective Agents; 0/Reactive Oxygen Species; 11062-77-4/Superoxides; 31098-42-7/WR 1065; EC Dismutase; EC dismutase 2

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