Document Detail


SOCS3 expression is inversely correlated with Pyk2 in non-small cell lung cancer and exogenous SOCS3 inhibits proliferation and invasion of A549 cells.
MedLine Citation:
PMID:  22722483     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
AIMS:: We have confirmed that suppressor of cytokine signalling 3 (SOCS3) is silenced and proline-rich tyrosine kinase 2 (Pyk2) is over-expressed in non-small cell lung cancer (NSCLC). The aim of this study was to investigate the correlation of SOCS3 and Pyk2 expression in NSCLC, and the effects of SOCS3 up-regulation on A549 cells. METHODS:: One hundred cases of NSCLC were detected for the expression of SOCS3 and Pyk2 by immunohistochemistry. The expression of SOCS3 and Pyk2 were also examined in human bronchial epithelial cells (HBE) and six lung cancer cell lines using Western blot and immunofluorescence staining. Then plasmid containing full-length SOCS3 was transfected into A549 cells to further investigate the effects of SOCS3 over-expression on proliferation, apoptosis and invasion of transfected cells, which were examined using MTT, flow cytometry and Transwell assays. RESULTS:: Our results showed a significant negative correlation between SOCS3 and Pyk2 in both NSCLC tissues and cell lines. Up-regulation of SOCS3 increased the apoptotic rates of transfected cells, while the numbers of proliferative and invasive cells were decreased. CONCLUSIONS:: Our data indicate that SOCS3 definitely plays roles in regulating Pyk2 expression, and up-regulation of SOCS3 could be an effective way to prevent the progression of NSCLC.
Authors:
Siyang Zhang; Wei Wang; Enhua Wang; Xueshan Qiu
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-6-19
Journal Detail:
Title:  Pathology     Volume:  -     ISSN:  1465-3931     ISO Abbreviation:  -     Publication Date:  2012 Jun 
Date Detail:
Created Date:  2012-6-22     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0175411     Medline TA:  Pathology     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
*Department of Pathology, The First Affiliated Hospital and College of Basic Medical Sciences, China Medical University †Center of Laboratory Technology and Experimental Medicine, China Medical University, Shenyang, China ‡these authors contributed equally.
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