Document Detail


SNR1 (INI1/SNF5) mediates important cell growth functions of the Drosophila Brahma (SWI/SNF) chromatin remodeling complex.
MedLine Citation:
PMID:  15454538     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
SNR1 is an essential subunit of the Drosophila Brahma (Brm) ATP-dependent chromatin remodeling complex, with counterparts in yeast (SNF5) and mammals (INI1). Increased cell growth and wing patterning defects are associated with a conditional snr1 mutant, while loss of INI1 function is directly linked with aggressive cancers, suggesting important roles in development and growth control. The Brm complex is known to function during G1 phase, where it appears to assist in restricting entry into S phase. In Drosophila, the activity of DmcycE/CDK2 is rate limiting for entry into S phase and we previously found that the Brm complex can suppress a reduced growth phenotype associated with a hypomorphic DmcycE mutant. Our results reveal that SNR1 helps mediate associations between the Brm complex and DmcycE/CDK2 both in vitro and in vivo. Further, disrupting snr1 function suppressed DmcycEJP phenotypes, and increased cell growth defects associated with the conditional snr1E1 mutant were suppressed by reducing DmcycE levels. While the snr1E1-dependent increased cell growth did not appear to be directly associated with altered expression of G1 or G2 cyclins, transcription of the G2-M regulator string/cdc25 was reduced. Thus, in addition to important functions of the Brm complex in G1-S control, the complex also appears to be important for transcription of genes required for cell cycle progression.
Authors:
Claudia B Zraly; Daniel R Marenda; Andrew K Dingwall
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Genetics     Volume:  168     ISSN:  0016-6731     ISO Abbreviation:  Genetics     Publication Date:  2004 Sep 
Date Detail:
Created Date:  2004-09-29     Completed Date:  2005-04-12     Revised Date:  2013-06-09    
Medline Journal Info:
Nlm Unique ID:  0374636     Medline TA:  Genetics     Country:  United States    
Other Details:
Languages:  eng     Pagination:  199-214     Citation Subset:  IM    
Affiliation:
Oncology Institute, Cardinal Bernardin Cancer Center, Stritch School of Medicine, Loyola University of Chicago, Maywood, Illinois 60153, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
CDC2-CDC28 Kinases / metabolism
Cell Cycle / physiology
Cell Cycle Proteins / genetics*
Cell Growth Processes / genetics,  physiology*
Chromatin Assembly and Disassembly / genetics*
Cyclin-Dependent Kinase 2
Drosophila Proteins / genetics,  metabolism,  physiology*
Drosophila melanogaster / genetics*
Electrophoresis, Polyacrylamide Gel
Fluorescent Antibody Technique
G1 Phase / genetics,  physiology
Gene Expression Regulation, Developmental*
Glutathione Transferase
Mutation / genetics
Phenotype*
Protein Tyrosine Phosphatases / metabolism
Reverse Transcriptase Polymerase Chain Reaction
Trans-Activators / genetics*
Transcription Factors / genetics,  metabolism,  physiology*
Wing / anatomy & histology
Chemical
Reg. No./Substance:
0/Cell Cycle Proteins; 0/Drosophila Proteins; 0/Snr1 protein, Drosophila; 0/Trans-Activators; 0/Transcription Factors; 0/brm protein, Drosophila; EC 2.5.1.18/Glutathione Transferase; EC 2.7.11.22/CDC2-CDC28 Kinases; EC 2.7.11.22/Cyclin-Dependent Kinase 2; EC 2.7.11.22/cdc2c protein, Drosophila; EC 3.1.3.48/Protein Tyrosine Phosphatases; EC 3.1.3.48/stg protein, Drosophila
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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