Document Detail


SNP genotyping with fluorescence polarization detection.
MedLine Citation:
PMID:  11933186     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
When a fluorescent molecule is excited by plane polarized light, the fluorescence emitted is also polarized. The degree of fluorescence polarization (FP) detected, under constant temperature and solvent viscosity, is proportional to the molecular weight of the dye molecule. By monitoring the FP of a fluorescent dye, one can detect significant changes in the molecular weight of the molecule without separation or purification. Because the size of the probe is altered in the course of a number of single nucleotide polymorphism (SNP) genotyping reactions, FP is therefore an excellent detection mechanism for these assays. Indeed, FP detection can be used in SNP genotyping with the primer extension TaqMan((R)) and Invader((R)) assays. Use of FP detection makes it possible to reduce the cost of TaqMan((R)) and Invader((R)) probes by abrogating the need for a fluorescence quencher. Moreover, inexpensive, unpurified, and unlabeled probes are used in the primer extension reaction with FP detection. As an end-point detection mechanism, FP detection is suitable for high-throughput SNP genotyping.
Authors:
Pui-Yan Kwok
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.; Review    
Journal Detail:
Title:  Human mutation     Volume:  19     ISSN:  1098-1004     ISO Abbreviation:  Hum. Mutat.     Publication Date:  2002 Apr 
Date Detail:
Created Date:  2002-04-04     Completed Date:  2002-08-30     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  9215429     Medline TA:  Hum Mutat     Country:  United States    
Other Details:
Languages:  eng     Pagination:  315-23     Citation Subset:  IM    
Copyright Information:
Copyright 2002 Wiley-Liss, Inc.
Affiliation:
Division of Dermatology and Department of Genetics, Washington University School of Medicine, St. Louis, Missouri, USA. kwok@genetics.wustl.edu
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MeSH Terms
Descriptor/Qualifier:
DNA Mutational Analysis / economics,  methods*
DNA Probes
Fluorescence Polarization / economics,  methods*
Genetic Testing / economics,  methods*
Genotype
Polymerase Chain Reaction
Polymorphism, Single Nucleotide / genetics*
Templates, Genetic
Grant Support
ID/Acronym/Agency:
R01-EY12557/EY/NEI NIH HHS
Chemical
Reg. No./Substance:
0/DNA Probes

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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