| SNP genotyping using multiplex single base primer extension assays. | |
| | |
MedLine Citation:
|
PMID: 19768606 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Single nucleotide polymorphisms (SNPs) are the most common form of polymorphisms present in the human genome. The single base primer extension (SBE) method is an effective and sensitive tool that can type over 30 known loci scattered throughout an organism's genome in a single reaction. It allows the typing of tetra-allelic SNPs and has been adapted to a broad range of analytical necessities: single-cell analysis, molecular diagnosis of monogenic diseases, forensic mitochondrial DNA analysis on highly degraded human remains, and high-throughput SNP screening for population studies. Every SBE-based assay will need customized optimization efforts that are generally proportional to the number of desired SNPs typed in a single reaction. This chapter offers a detailed outline on which to base the design and optimization of any multiplex SBE assay that can then be tailored to the analytical conditions that characterize each specific application. |
| | |
Authors:
|
Daniele Podini; Peter M Vallone |
Related Documents
:
|
18838176 - Susceptibility to guillain-barré syndrome is not associated with cd1a and cd1e gene po... 15867866 - Polymorphisms in the 5' region of the cd14 gene are associated with eczema in young chi... 20184506 - Single-nucleotide polymorphisms in the fsh receptor gene and ovarian performance: futur... 17277236 - A population-based association study of snps of gstp1, mnsod, gpx2 and barrett's esopha... 19013876 - Novel foxc2 missense mutation identified in patient with lymphedema-distichiasis syndro... 10442986 - Applicability of bovine microsatellite markers for population genetic studies on africa... |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: Methods in molecular biology (Clifton, N.J.) Volume: 578 ISSN: 1940-6029 ISO Abbreviation: Methods Mol. Biol. Publication Date: 2009 |
Date Detail:
|
Created Date: 2009-09-21 Completed Date: 2009-12-30 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 9214969 Medline TA: Methods Mol Biol Country: United States |
Other Details:
|
Languages: eng Pagination: 379-91 Citation Subset: IM |
Affiliation:
|
Department of Forensic Sciences, The George Washington University, Washington, DC, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Base Pairing
/
genetics* Biological Assay / methods* DNA Primers / metabolism* DNA, Mitochondrial / genetics Genotype Humans Open Reading Frames / genetics Polymorphism, Single Nucleotide / genetics* |
| Chemical | |
Reg. No./Substance:
|
0/DNA Primers; 0/DNA, Mitochondrial |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Putting the invader(r) assay to work: laboratory application and data management.
Next Document: High-throughput SNP detection based on PCR amplification on magnetic nanoparticles using dual-color ...