Document Detail

SNP detection in Na/K ATP-ase gene α(1) subunit of bisexual and parthenogenetic Artemia strains by RFLP screening.
MedLine Citation:
PMID:  21429125     Owner:  NLM     Status:  In-Data-Review    
In order to find a marker for differentiating between a bisexual and a parthenogenetic Artemia strain, Exon-7 of the Na/K ATPase α(1) subunit gene was screened by RFLP technique. The results revealed a constant synonymous SNP (single nucleotide polymorphism) in digestion by the Tru1I enzyme that was consistent with these two types of Artemia. This SNP was identified as an accurate molecular marker for discrimination between bisexual and parthenogenetic Artemia. According to the Nei's genetic distance (1973), the lowest genetic distance was found between individuals from Artemia urmiana Günther 1890 and parthenogenetic populations, making the described marker the first marker to easily distinguish between these two cooccurring species.
R Manaffar; S Zare; N Agh; N Abdolahzadeh; S Soltanian; P Sorgeloos; P Bossier; G VAN Stappen
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Publication Detail:
Type:  Journal Article     Date:  2010-08-18
Journal Detail:
Title:  Molecular ecology resources     Volume:  11     ISSN:  1755-0998     ISO Abbreviation:  Mol Ecol Resour     Publication Date:  2011 Jan 
Date Detail:
Created Date:  2011-03-24     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101465604     Medline TA:  Mol Ecol Resour     Country:  England    
Other Details:
Languages:  eng     Pagination:  211-4     Citation Subset:  IM    
Copyright Information:
© 2010 Blackwell Publishing Ltd.
Artemia and Aquatic Animals Research Institute, Urmia University, PO Box 165, Urmia, Iran Department of Biology, Faculty of Science, Urmia University, PO Box 165, Urmia, Iran Aquatic Animals Health & Disease Department, School of Veterinary Medicine, Shiraz University, Shiraz, 71345-1731, Iran Laboratory of Aquaculture & Artemia Reference Center, Ghent University, Rozier 44, B-9000 Gent, Belgium.
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