Document Detail

SNP, ARMS and SSH authentication of medicinal Dendrobium officinale KIMURA et MIGO and application for identification of Fengdou drugs.
MedLine Citation:
PMID:  18379039     Owner:  NLM     Status:  MEDLINE    
Dried stems of Dendrobium officinale have been used as crude drugs in traditional Chinese medicine (TCM) with good tonic efficacy. Sequences of chloroplast, nuclear and mitochondria genes and the method of genomic DNA (gDNA) suppression subtraction hybridization (SSH) were used to authenticate different populations during the process of good agriculture practice (GAP) and crude drug quality control. Six populations could be authenticated successfully by nine single sucleotide polymorphism (SNP) sites and six pairs of diagnostic primers for amplification refractory mutation system (ARMS) were also designed to identify six populations on the basis of single nucleotide polymorphism (SNPs). The remainder two populations (JSR, GGL) with the same sequences could be authenticated by SSH. One population-specific fragment was obtained by SSH and a pair of specific primers (SSH-JB01, SSH-JB02) on the specific sequence was designed to authenticate GGL population from the other populations tested. As the resultants were population-specific, the botanic origins of fifty "Fengdou" drug samples from markets could be classified. It is evident that the combined methods provide a high throughput and reliable approach for identification of D. officinale plants and "Fengdou" drugs.
Ge Ding; Daizhen Zhang; Zhenyu Feng; Wenjing Fan; Xiaoyu Ding; Xuexia Li
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biological & pharmaceutical bulletin     Volume:  31     ISSN:  0918-6158     ISO Abbreviation:  Biol. Pharm. Bull.     Publication Date:  2008 Apr 
Date Detail:
Created Date:  2008-04-01     Completed Date:  2008-05-12     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9311984     Medline TA:  Biol Pharm Bull     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  553-7     Citation Subset:  IM    
Jiangsu Provicinal Key Laboratory for Biodiversity and Biotechnology, College of Life Sciences, Nanjing Normal University, No. 1, Wenyuan Road, Nanjing 210046, China.
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MeSH Terms
Cloning, Molecular
DNA Primers
DNA, Plant / genetics
Dendrobium / chemistry*,  genetics
Drugs, Chinese Herbal / chemistry*,  standards
Introns / genetics
Mitochondria / metabolism
Mutation / genetics
Nucleic Acid Hybridization
Polymorphism, Single Nucleotide
Reg. No./Substance:
0/DNA Primers; 0/DNA, Plant; 0/Drugs, Chinese Herbal

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