| SNEV overexpression extends the life span of human endothelial cells. | |
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MedLine Citation:
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PMID: 16388800 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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In a recent screening for genes down regulated in replicatively senescent human umbilical vein endothelial cells (HUVECs), we have isolated the novel protein SNEV. Since then SNEV has proven as a multifaceted protein playing a role in pre-mRNA splicing, DNA repair, and the ubiquitin/proteosome system. Here, we report that SNEV mRNA decreases in various cell types during replicative senescence, and that it is increased in various immortalized cell lines, as well as in breast tumors, where SNEV transcript levels also correlate with the survival of breast cancer patients. Since these mRNA profiles suggested a role of SNEV in the regulation of cell proliferation, the effect of its overexpression was tested. Thereby, a significant extension of the cellular life span was observed, which was not caused by altered telomerase activity or telomere dynamics but rather by enhanced stress resistance. When SNEV overexpressing cells were treated with bleomycin or bleomycin combined with BSO, inducing DNA damage as well as reactive oxygen species, a significantly lower fraction of apoptotic cells was found in comparison to vector control cells. These data suggest that high levels of SNEV might extend the cellular life span by increasing the resistance to stress or by improving the DNA repair capacity of the cells. |
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Authors:
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Regina Voglauer; Martina Wei-Fen Chang; Brigitta Dampier; Matthias Wieser; Kristin Baumann; Thomas Sterovsky; Martin Schreiber; Hermann Katinger; Johannes Grillari |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2006-01-04 |
Journal Detail:
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Title: Experimental cell research Volume: 312 ISSN: 0014-4827 ISO Abbreviation: Exp. Cell Res. Publication Date: 2006 Apr |
Date Detail:
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Created Date: 2006-03-20 Completed Date: 2006-05-16 Revised Date: 2007-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0373226 Medline TA: Exp Cell Res Country: United States |
Other Details:
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Languages: eng Pagination: 746-59 Citation Subset: IM |
Affiliation:
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Institute of Applied Microbiology, Department of Biotechnology, University of Natural Resources and Applied Life Sciences Muthgasse 18, A-1190 Vienna, Austria. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adult Aged Aged, 80 and over Breast Neoplasms / diagnosis, genetics* Cell Aging / genetics*, physiology Cell Proliferation Cells, Cultured DNA Damage / physiology DNA Repair / physiology DNA Repair Enzymes Endothelial Cells / cytology*, physiology* Female Gene Expression Regulation* Humans Middle Aged Nuclear Proteins / genetics*, metabolism RNA, Messenger / genetics, metabolism Reverse Transcriptase Polymerase Chain Reaction |
| Chemical | |
Reg. No./Substance:
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0/Nuclear Proteins; 0/RNA, Messenger; EC 6.5.1.-/DNA Repair Enzymes; EC 6.5.1.-/PRPF19 protein, human |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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