Document Detail

SNAT2 silencing prevents the osmotic induction of transport system A and hinders cell recovery from hypertonic stress.
MedLine Citation:
PMID:  15922329     Owner:  NLM     Status:  MEDLINE    
Under hypertonic conditions the induction of SLC38A2/SNAT2 leads to the stimulation of transport system A and to the increase in the cell content of amino acids. In hypertonically stressed human fibroblasts transfection with two siRNAs for SNAT2 suppressed the increase in SNAT2 mRNA and the stimulation of system A transport activity. Under the same condition, the expansion of the intracellular amino acid pool was significantly lowered and cell volume recovery markedly delayed. It is concluded that the up-regulation of SNAT2 is essential for the rapid restoration of cell volume after hypertonic stress.
Elena Bevilacqua; Ovidio Bussolati; Valeria Dall'Asta; Francesca Gaccioli; Roberto Sala; Gian C Gazzola; Renata Franchi-Gazzola
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  FEBS letters     Volume:  579     ISSN:  0014-5793     ISO Abbreviation:  FEBS Lett.     Publication Date:  2005 Jun 
Date Detail:
Created Date:  2005-06-17     Completed Date:  2005-08-08     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0155157     Medline TA:  FEBS Lett     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  3376-80     Citation Subset:  IM    
Sezione di Patologia Generale e Clinica, Dipartimento di Medicina, Sperimentale, Università degli Studi di Parma, Via Volturno 39, 43100 Parma, Italy.
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MeSH Terms
Amino Acid Transport System A / antagonists & inhibitors*,  genetics*,  physiology*
Amino Acids / metabolism
Cell Size
Fibroblasts / drug effects
Osmotic Pressure
RNA Interference*
RNA, Messenger / analysis,  metabolism
RNA, Small Interfering / genetics,  pharmacology
Saline Solution, Hypertonic / pharmacology
Reg. No./Substance:
0/Amino Acid Transport System A; 0/Amino Acids; 0/RNA, Messenger; 0/RNA, Small Interfering; 0/SLC38A2 protein, human; 0/Saline Solution, Hypertonic

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