Document Detail


SMU.746-SMU.747, a putative membrane permease complex, is involved in aciduricity, acidogenesis, and biofilm formation in Streptococcus mutans.
MedLine Citation:
PMID:  24142257     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Dental caries induced by Streptococcus mutans is one of the most prevalent chronic infectious diseases worldwide. The pathogenicity of S. mutans relies on the bacterium's ability to colonize tooth surfaces and survive a strongly acidic environment. We performed an ISS1 transposon mutagenesis to screen for acid-sensitive mutants of S. mutans and identified an SMU.746-SMU.747 gene cluster that is needed for aciduricity. SMU.746 and SMU.747 appear to be organized in an operon and encode a putative membrane-associated permease. SMU.746- and SMU.747-deficient mutants showed a reduced ability to grow in acidified medium. However, the short-term or long-term acid survival capacity and F1F0 ATPase activity remained unaffected in the mutants. Furthermore, deletion of both genes did not change cell membrane permeability and the oxidative and heat stress responses. Growth was severely affected even with slight acidification of the defined medium (pH 6.5). The ability of the mutant strain to acidify the defined medium during growth in the presence of glucose and sucrose was significantly reduced, although the glycolysis rate was only slightly affected. Surprisingly, deletion of the SMU.746-SMU.747 genes triggered increased biofilm formation in low-pH medium. The observed effects were more striking in a chemically defined medium. We speculate that the SMU.746-SMU.747 complex is responsible for amino acid transport, and we discuss its possible role in colonization and survival in the oral environment.
Authors:
Jaroslaw E Król; Saswati Biswas; Clay King; Indranil Biswas
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2013-10-18
Journal Detail:
Title:  Journal of bacteriology     Volume:  196     ISSN:  1098-5530     ISO Abbreviation:  J. Bacteriol.     Publication Date:  2014 Jan 
Date Detail:
Created Date:  2013-12-09     Completed Date:  2014-02-11     Revised Date:  2014-07-01    
Medline Journal Info:
Nlm Unique ID:  2985120R     Medline TA:  J Bacteriol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  129-39     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Bacterial Proteins / genetics,  metabolism*
Biofilms / growth & development*
Carboxylic Acids / metabolism*
Cell Membrane Permeability / drug effects
Culture Media / chemistry
DNA Transposable Elements
Gene Deletion
Glucose / metabolism
Hydrogen-Ion Concentration
Membrane Transport Proteins / genetics,  metabolism*
Microbial Viability / drug effects
Multigene Family
Mutagenesis, Insertional
Operon
Streptococcus mutans / drug effects,  genetics,  metabolism,  physiology*
Sucrose / metabolism
Grant Support
ID/Acronym/Agency:
DE021664/DE/NIDCR NIH HHS
Chemical
Reg. No./Substance:
0/Bacterial Proteins; 0/Carboxylic Acids; 0/Culture Media; 0/DNA Transposable Elements; 0/Membrane Transport Proteins; 57-50-1/Sucrose; IY9XDZ35W2/Glucose
Comments/Corrections

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