| SLC37A1 and SLC37A2 are phosphate-linked, glucose-6-phosphate antiporters. | |
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MedLine Citation:
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PMID: 21949678 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Blood glucose homeostasis between meals depends upon production of glucose within the endoplasmic reticulum (ER) of the liver and kidney by hydrolysis of glucose-6-phosphate (G6P) into glucose and phosphate (P(i)). This reaction depends on coupling the G6P transporter (G6PT) with glucose-6-phosphatase-α (G6Pase-α). Only one G6PT, also known as SLC37A4, has been characterized, and it acts as a P(i)-linked G6P antiporter. The other three SLC37 family members, predicted to be sugar-phosphate:P(i) exchangers, have not been characterized functionally. Using reconstituted proteoliposomes, we examine the antiporter activity of the other SLC37 members along with their ability to couple with G6Pase-α. G6PT- and mock-proteoliposomes are used as positive and negative controls, respectively. We show that SLC37A1 and SLC37A2 are ER-associated, P(i)-linked antiporters, that can transport G6P. Unlike G6PT, neither is sensitive to chlorogenic acid, a competitive inhibitor of physiological ER G6P transport, and neither couples to G6Pase-α. We conclude that three of the four SLC37 family members are functional sugar-phosphate antiporters. However, only G6PT/SLC37A4 matches the characteristics of the physiological ER G6P transporter, suggesting the other SLC37 proteins have roles independent of blood glucose homeostasis. |
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Authors:
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Chi-Jiunn Pan; Shih-Yin Chen; Hyun Sik Jun; Su Ru Lin; Brian C Mansfield; Janice Y Chou |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Intramural Date: 2011-09-20 |
Journal Detail:
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Title: PloS one Volume: 6 ISSN: 1932-6203 ISO Abbreviation: PLoS ONE Publication Date: 2011 |
Date Detail:
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Created Date: 2011-09-28 Completed Date: 2012-03-01 Revised Date: 2012-05-07 |
Medline Journal Info:
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Nlm Unique ID: 101285081 Medline TA: PLoS One Country: United States |
Other Details:
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Languages: eng Pagination: e23157 Citation Subset: IM |
Affiliation:
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Section on Cellular Differentiation, Program in Developmental Endocrinology and Genetics, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland, United States of America. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Antiporters / genetics, metabolism* Biological Transport Blotting, Western COS Cells Cercopithecus aethiops Endoplasmic Reticulum / metabolism* Gene Expression Profiling Glucose / metabolism Glucose-6-Phosphate / metabolism, pharmacokinetics Humans Intestines / metabolism Kidney / metabolism Liver / metabolism Membrane Transport Proteins / genetics, metabolism* Mice Microscopy, Fluorescence Monosaccharide Transport Proteins / genetics, metabolism* Pancreas / metabolism Phosphates / metabolism* Proteolipids / metabolism Reverse Transcriptase Polymerase Chain Reaction |
| Chemical | |
Reg. No./Substance:
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0/Antiporters; 0/Membrane Transport Proteins; 0/Monosaccharide Transport Proteins; 0/Phosphates; 0/Proteolipids; 0/Slc37a2 protein, human; 0/glucose 6-phosphate(transporter); 0/proteoliposomes; 50-99-7/Glucose; 56-73-5/Glucose-6-Phosphate; 87434-91-1/SLC37A1 protein, human |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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