| SK-HEP cells and lentiviral vector for production of human recombinant factor VIII. | |
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MedLine Citation:
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PMID: 22488441 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Hemophilia A is caused by a deficiency in coagulation factor VIII. Recombinant factor VIII can be used as an alternative although it is unavailable for most patients. Here, we describe the production of a human recombinant B-domain-deleted FVIII (rBDDFVIII) by the human cell line SK-HEP-1, modified by a lentiviral vector rBDDFVIII was produced by recombinant SK-HEP cells (rSK-HEP) at 1.5-2.1 IU/10(6) in 24 h. The recombinant factor had increased in vitro stability when compared to commercial pdFVIII. The functionality of rBDDFVIII was shown by its biological activity and by tail-clip challenge in hemophilia A mice. The rSK-HEP cells grew in a scalable system and produced active rBDDFVIII, indicating that this platform production can be optimized to meet the commercial production scale needs. |
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Authors:
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Nathalia Gonsales da Rosa; Kamilla Swiech; Virgínia Picanço-Castro; Elisa Maria de Sousa Russo-Carbolante; Mario Abreu Soares Neto; Andrielle de Castilho-Fernandes; Vitor Marcel Faça; Aparecida Maria Fontes; Dimas Tadeu Covas |
Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-4-10 |
Journal Detail:
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Title: Biotechnology letters Volume: - ISSN: 1573-6776 ISO Abbreviation: - Publication Date: 2012 Apr |
Date Detail:
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Created Date: 2012-4-10 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8008051 Medline TA: Biotechnol Lett Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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Center for Cell Therapy and Regional Blood Center of Ribeirão Preto, University of São Paulo (USP), Ribeirão Preto, 14051-140, Brazil, nathaliagrosa@gmail.com. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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