| SIRT1 regulation of apoptosis of human chondrocytes. | |
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MedLine Citation:
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PMID: 19714620 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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OBJECTIVE: SIRT1 is known to inhibit apoptosis and to promote survival of various types of cells. However, the roles of SIRT1 in apoptosis of human chondrocytes have never been reported. We undertook this study to investigate the relationship of SIRT1 to apoptosis of human chondrocytes, which is a characteristic feature of osteoarthritis (OA). METHODS: The expression of SIRT1 in human chondrocytes was examined by reverse transcription-polymerase chain reaction, immunoblotting, and immunohistology of human cartilage samples. The expression of SIRT1 under catabolic, mechanical, and nutritional stresses was investigated by immunoblotting. To examine the effect of SIRT1 on apoptosis, SIRT1 was inhibited by small interfering RNA (siRNA) and activated by resveratrol during nitric oxide (NO)-induced apoptosis. TUNEL staining and immunoblotting of cleaved poly(ADP-ribose) polymerase (PARP) were performed to detect apoptosis. To examine the mechanisms of apoptosis, we used immunoblotting to determine the levels of cleaved caspases and mitochondria-related apoptotic signaling proteins, Bax and Bcl-2, in the mitochondrial fraction. RESULTS: SIRT1 expression was confirmed in human chondrocytes and human cartilage samples. All catabolic, mechanical, and nutritional stresses inhibited SIRT1 expression. SIRT1 inhibition by siRNA for SIRT1 increased the percentage of TUNEL-positive cells and increased the amounts of cleaved PARP and cleaved caspases 3 and 9 induced by NO. In contrast, treatment with resveratrol decreased the percentage of TUNEL-positive cells and decreased the amounts of cleaved PARP and cleaved caspases 3 and 9 induced by NO. Furthermore, in the mitochondrial fraction, SIRT1 inhibition by siRNA for SIRT1 increased the amount of Bax but reduced the amount of Bcl-2, while resveratrol reduced the amount of Bax but increased the amount of Bcl-2. CONCLUSION: These results indicate that SIRT1 regulates apoptosis in human chondrocytes through the modulation of mitochondria-related apoptotic signals. Further research on SIRT1 might contribute to resolving the pathogenesis of OA. |
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Authors:
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Koji Takayama; Kazunari Ishida; Takehiko Matsushita; Norifumi Fujita; Shinya Hayashi; Ken Sasaki; Katsumasa Tei; Seiji Kubo; Tomoyuki Matsumoto; Hiroyuki Fujioka; Masahiro Kurosaka; Ryosuke Kuroda |
Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Arthritis and rheumatism Volume: 60 ISSN: 0004-3591 ISO Abbreviation: Arthritis Rheum. Publication Date: 2009 Sep |
Date Detail:
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Created Date: 2009-09-21 Completed Date: 2009-10-19 Revised Date: 2009-11-19 |
Medline Journal Info:
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Nlm Unique ID: 0370605 Medline TA: Arthritis Rheum Country: United States |
Other Details:
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Languages: eng Pagination: 2731-40 Citation Subset: AIM; IM |
Affiliation:
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Department of Orthopaedic Surgery, Kobe University Graduate School of Medicine, 70501 Kusunoki-cho, Kobe, Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adult Apoptosis / drug effects, physiology* Benzamides / pharmacology Case-Control Studies Caspase 3 / metabolism Caspase 9 / metabolism Cells, Cultured Chondrocytes / metabolism*, pathology* Enzyme Inhibitors / pharmacology Humans Male Naphthols / pharmacology Niacinamide / pharmacology Nitric Oxide / pharmacology Osteoarthritis, Knee / metabolism*, pathology* Poly(ADP-ribose) Polymerases / metabolism Proto-Oncogene Proteins c-bcl-2 / metabolism RNA, Small Interfering / pharmacology Sirtuin 1 Sirtuins / antagonists & inhibitors, metabolism* Stilbenes / pharmacology bcl-2-Associated X Protein / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Benzamides; 0/Enzyme Inhibitors; 0/Naphthols; 0/Proto-Oncogene Proteins c-bcl-2; 0/RNA, Small Interfering; 0/Stilbenes; 0/bcl-2-Associated X Protein; 0/sirtinol; 10102-43-9/Nitric Oxide; 501-36-0/resveratrol; 98-92-0/Niacinamide; EC 2.4.2.30/Poly(ADP-ribose) Polymerases; EC 3.4.22.-/Caspase 3; EC 3.4.22.-/Caspase 9; EC 3.5.1.-/SIRT1 protein, human; EC 3.5.1.-/Sirtuin 1; EC 3.5.1.-/Sirtuins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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